The Expression Of MiR-155 In Hepatocellular Carcinoma And Its Impact In Cell Invasion In HCC

Objective:To investigate the expression of micro RNA-155 in human hepatocellular carcinoma( HCC) cells and tissue samples.Then further explore the physiological functions of mi R-155 in cell growth and and explore its relationship with invasion and metastasis of HCC cells, The ultimate goal is to provide an exciting new target for the diagnosis and treatment of HCC.Methods:1、HCC cell lines Hu H7 and Hep G2 were chosen, the normal human normal liver cells HL-7702 was taken as control. In addition, we collected 30 cases of HCC specimens and 12 cases of normal liver tissue samples. Using q RT-PCR to detect the expression of mi R-155 in HCC cells and tissue specimens. 2 、mi R-155, mi R-155 negative control were transfected to Hu H7 cells which expressed lower levels of mi R-155; mi R-155 inhibitor, mi R-155 inhibitor negative control were transfected to Hep G2 cells which expressed higher levels of mi R-155. Then, we evaluated the cell proliferation, cycle, migration, and invasion using MTT, cell cycle assay,wound healing, and transwell assay in HCC cells.3 、 Using q RT-PCR to detect the expression of epithelial-mesenchymal transition(EMT) phenotype associated factor gene and its associated protein expression by Western blot, and analyze the relationship between mi R-155 and EMT. 4、Using q RT-PCR to detect the expression of mi R-155 and the relationship between tumor size and distant metastasis, and explore the impact in HCC occurrence, development and prognosis.Results:1、mi R-155 expression in HCC cell was significantly higher than the normal liver cell HL-7702,(P <0.05), and Hep G2 cell line was higher,Hu H7 cell lines was lower.and the expression of mi R-155 in HCC tissues was significantly higher than in adjacent and normal tissues(P <0.05). 2、 mi R-155 gain of function promotes whereas knockdown of mi R-155 reduces cell migration and invasion. But it had no in?uence oncell proliferation and cell cycle.3 、 the m RNA and protein expression of epithelial phenotype CDH1 was reduced,while the mesenchymal phenotype of snai1 and zeb1 were increasing after Hu H7 cell line transfected with mi R-155.while Hep G2 cell line transfected with mi R-155 inhibitor, showed the opposite result.4 、 Statistical analysis clinical tissues showed that mi R-155 has nothing to do with the size of HCC,but significantly associated with distant metastasis.Conclusion : In summary, we have characterized that mi R-155 is commonly and markedly up-regulated in HCC, and is able to promote the invasion and metastasis ability of HCC by promoting EMT. Thus, mi R-155 could become a new prognostic indicators for monitoring of HCC.