Correlation Between Gene Polymorphisms Of Fn And Renal Calcium Oxalate Stones Susceptibility In The Uygur Nationality:a Case-control Study

Objective:To investigate the correlation between gene polymorphisms of Fn g-ene and renal calcium oxalate stones susceptibility in the Uygur nationality. Meth-od:129 patients with calciu-m oxalate stones (case group) and 94 healthy people (c ontrol group) were enrolled in our study, peripheral blood was collected and geno-mic DNA were extracted from peripheral blood, polymerase chain reaction restricti-on fragment length polymorphism (PCR-RFLP) was performed to analysis polymor-phisms rs6725958, rs10202709 and rs35343655 of Fn, compare association between different genetypes and calcium oxalate stones susceptibility, and made a compreh-ensive analysis of these SNP locies distribution of alleles in Xinjiang Uygur calciu-m oxalate stones patients and healthy people. ②Immunohistochemistry was used to detect the expression of Fn in kidney tissue between the case group and the con-trol group,then make a comprehensive statistical analysis of test results. Results:Th-e results of genetype and alleles of Fn gene reveals that the distributation of three locis goes with Hardy-Weinberg equilibrium,there has no not statistically difference between the three gene sites,Immunohistochemistry results reveals that calcium oxa-late stones group has a higher expression rate, statistically difference was found b-etween t-he two groups. Conclusions: ①rs6725958, rs10202709 and rs35343655 p-olymorphisms of Fn gene has no stasitically association with calcium oxalate stone-s susceptibility and may not be the predisposing genes of calcium oxalate stones.② Fn protein in renal cortex were significantly different in both groups,suggesting that the some unfound molecular mechanisms of Fn gene to inhibit the formation of c-alcium oxalate stones, our results may related to race and sample size, and still n -eed further study.