Study Of HLA DQB1 Allele, Immune Cells For Cervical Cancer In Clinical Characteristics And The Recent Curative Effect

Objective:Study on HLA-DQB1 allele with cervical cancer in xinjiang, to look for alleles related cervical cancer, to explore the variation of immune cells subpopulation in peripheral blood. Methods:140 patients with cervical cancer (CC) and 159 healthy controls were selected, HLA-DQB1 alleles were detected by polymerase chain reaction/sequence specific primers, and immune cells population were analysed by flow cytometry. According to different clinical factors and short-term effect, allele frequency and immune cells population were compared. Results:(1) HLA-DQB1*O2 allele frequency of CC was 25.7%, which was higher than the healthy controls 17.0%(P<0.05). HLA-DQB1*03 allele frequency of CC was 36.8%, which was lower than the healthy controls 48.7%(P<0.05). HLA-DQB1*04, HLA-DQB1-05 and HLA-DQB1-06 had no statistical differences between the two groups. Accroding to age or expression of HPV16 grouping, there were no statistical differences in all allele frequency (P>0.05). HLA-DQB1*03 allele frequency in the group of (CR+PR) was higher than in the group of (SD+PD) (P<0.05). (2) The proportions of CD3+ T cells, CD8+ T cells and NK cells for cervical cancer were lower than the healthy controls. CD4+ and CD4+/CD8+T cells were higher than healthy controls (P<0.05). There were no statistical difference for proportions of immune cells between uighurs and han Chinese. Accroding to age or clinical stages grouping, there were no statistical differences in all immune cells population (P>0.05). The proportion of CD4+T cells in HPV16 positive group was higher than in HPV16 negative group, and reversed in NK cells (P<0.05). The proportion of immune cells subpopulation began return to normal at the time of lm after chemoradiotherapy in CC, and at the time of 4ms after chemoradiotherapy, the proportion regain to the level of healthy control. Conclusion:(1) HLA-DQB1*02 is possibly susceptibility allele for cervical cancer, but HLA-DQB1*03 maybe is protective allele, and it is probably associated with short-term effect. (2) There are differences in immune cells subpopulation between CC and healthy control. Immune cells show regular changes after chemoradiotherapy. Chemoradiotherapy could improve the immune function.