| Objective:To observe the differential protein expression profile in ASCI(acute spinal cord injury) after intrathecal injection of high-dose methylprednisolone in rats model of ASCI.Methods:Eight Sprague-Dawley rats model of ASCI were randomly divided into two groups, after intrathecal injection of methylprednisolone 7.5 mg/kg at 0 and 8 hours. The injured spinal cord tissue was harvested after 24 hours of injection. The differentially expressed proteins and nerve regeneration-related differential proteins were analyzed using iTRAQ (isobaric tags for relative and absolute quantitation) technical analysis. Results:Totally 87 differentially expressed proteins were identified in this study. Compared with 0 hour group, there were 43 up-regulated differential proteins and 44 down-regulated differential proteins.There were 18 differential proteins which related to neural regeneration, the number of up-regulation proteins was 8 and down-regulation proteins was 10. OMgp(Oligodendrocyte-myelin glycoprotein) was a potential neural axon growth inhibitory factor, OMgp specifically binds with NgR/P75/TROY/Lingo-1 to form receptor complexes, and activate RhoA through the second messenger cAMP, then inhibiting the collapse of axon growth cone.Conclusion:Identified differential proteins and nerve regeneration-related differential proteins after ASCI in rats, were respectively analyzed for protein database retrieval and protein function, its expression may serve as the indicator of monitoring nerve regeneration after ASCI. |
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