Study On Uygur Medicine Diaphragma Juglandis Fructus Chemical Composition,quality Standard And Activity

Objective: To study Uygur Medicine diaphragma juglandis fructus chemical composition; To establish a quality control method of Uygur Medicine diaphragma juglandis fructus; To explore the Uygur Medicine diaphragma juglandis fructus antioxidant activity and anti-cancer activity. Methods: 1. By using the solvent method, silica gel column chromatography, and semi-preparative reversed-phase silica gel liquid phase Uygur medicine walnut wood distracted 95% ethanol extract of petroleum ether extract, chloroform and ethyl acetate extract was subjected to chemical Isolation and purification of components, by using modern spectroscopic techniques(1H-NMR, 13C-NMR) of the compounds were isolated structure analysis identified; 2. Uygur Medicine diaphragma juglandis fructus powder microscopic identification; measured batches Uygur Medicine diaphragma juglandis fructus moisture, ash and extract; establish Uygur Medicine diaphragma juglandis fructus TLC method; Using UV determined batches content of Uygur Medicine diaphragma juglandis fructus total saponins and total flavonoids;3. Use of UV method combined with the Bio-TCL determination of antioxidant capacity of various polar extract. 4. The use of cervical cancer xenograft model U27 explore the anti-cancer activity of Uygur Medicine diaphragma juglandis fructus water extract and ethanol extracts. Result: 1. Uygur Medicine diaphragma juglandis fructus petroleum ether extract, chloroform and ethyl acetate extracts were isolated and purified, six compounds were obtained, respectively, 2-ethoxy juglone(I), β-sitosterol(II), iso sakuranetin(III), quercetin(IV), naringenin(V), gallic acid(VI). 2. Microscopic characteristics show Uygur medicine diaphragma juglandis fructus powder contains numerous catheters, main thread catheters, textured catheters and bordered pits catheter, also contain wood fibers, stone cells and wood parenchyma cells. Established method for the determination of Uygur Medicine diaphragma juglandis fructus of total saponins and flavonoids, and 11 batches of medicines were measured, the results of the average content of total saponins 16.323 mg·g-1, the average content of total flavonoids 71.175mg·g-1. 3.The results of anti-oxidation Vc, petroleum ether extract, water, sites, n-butanol extract, ethyl acetate extract and chloroform extracts IC50 were 0.0155 mg ml-1, 0.488mg·ml-1, 0.0499mg·ml-1, 0.0211mg·ml-1, 0.0092g·ml-1, 0.147mg·ml-1. 4. Uygur Medicine diaphragma juglandis fructus water extract and alcohol extract can inhibit the growth of mouse sarcoma tumor inhibition rate was 48.8% and 30.5%, HE staining of tumor tissue tumor cell necrosis, especially water extract large group of tumor cells necrosis, nuclear disappeared, interstitial edema, occasionally scattered inflammatory cells. Conclusion: 1. This is the first distraction from the Uygur Medicine diaphragma juglandis fructus isolated compound III and V. 2. Uygur Medicine diaphragma juglandis fructus provides a method of quality assessment; 3. Uygur Medicine diaphragma juglandis fructus ethyl acetate extract has good resistance to oxidation; 4. In vivo tests showed that the water extract and ethanol extracts of Uygur Medicine diaphragma juglandis fructus have certain anti-cancer effect and aqueous extract stronger role; This results for the Uygur Medicine diaphragma juglandis fructus subsequent exploitation provide a reference.