The Effects And Mechanism Of PRMT2 Gene Over-expression On Proliferation In Human Thyroid Cancer SW579 Cells

Objective:To investigate the effects and mechanisms of PRMT2 gene over-expression on cell proliferation in thyroid carcinom SW579 cells.Methods:1) The thyroid squamous cell carcinoma SW579 was used for this study. The slow virus infection method was used to transfect LV-PRMT2(4952-1) into SW579 cells.The puromycin was applied to screen the stable PRMT2 over-expression cell lines.Whether the transfection was successful was verified by using Western blot to detect the expression of PRMT2-3Flag fusion protein. After that the stable PRMT2 over-expression cells were picked for the following experiments. 2) The effect of PRMT2 over-expression on the proliferation in SW579 cells was determined by MTT and the clone formation experiment was also performed to detect the effect on ability of clone formation.Meanwhile,the protein expression of PRMT2, p-AKT, p-GSK-3β, CCND1 were detected by Western blot; and the interaction between TRβ and PRMT2 was determined by immune-coprecipitation.Results: 1. The stable overexpression of PRMT2 in SW579 cellscan be obtained from LV-PRMT2(4952-1) transfection and screened bytetracycline.2. The results of MTT experiment and clone formation assay showed that over-expressed PRMT2 can inhibit the proliferation of SW579 cells.3. Expressing of P-Akt, P-GSK-3β, CCND1 in SW579 cell were down-regulated by over-expression of PRMT2, while make no change in TRβ.4.Co-immunprecipitation showed the PRMT2 interacted with the TRβ in SW579 cells.Conclusions: 1.PRMT2 over-expression may inhibit the proliferation of thyroid cancer sw579 cells may via the regulation of Akt/GSK-3β/CCND1 pathway.2.There is an interaction between TRβ and PRMT2 in SW579 cells.