| Objective: In this study, an ARDS model of rats exposed to Dedevap is established. Changes of H2S/CSE system and its influence on NF-κB p65 and ICAM-1 in lungs are observed.Methods:1. To divide 48 male SD rats into two groups randomly: a control group, accepted 2ml normal saline; a experimental group, given 2ml dedevap(40mg/kg). 12 rats, 6 ones from the control group and the others from the experimental group, are killed at designed observing points(3h, 6h, 12 h, 24h).2. To the 12 rats, the following data are measured: the Pa O2, ratio of dry/wet lung, content of tatal protein in BALF, the level of H2 S in plasma, the activity of CSE, the pathological changes of the rats’ lung tissue, intercilluar adhesion molecule-1( ICAM-1) m RNA in lung tissue by RT-PCR, the level of NF-κB p65 by WB.Result:1. Results on gross examination:for the rats of control group, lung tissues are normal; but for experimental-group rats, not--- the tissues become congested in 3h, and worst in 6h.2. Microscopic examination: for the rats in the experimental group, the lung tissues are injured in 3h, not worse any more in 6h, and better gradually from 6h to 12h: for those in the control group, the tissues are normal.3. Pathological scores of lung injure: compared with the control group’ scores, the scores raises much higher. The higher the scores are, the worse the lung injure is. The scores become higher and higher with time, the worst at 6th hour. Then the values go down from 12 th to 24 th hour, yet much higher than the normal ones.4. Pa O2 changes and its relation to lung injury: for the rats of the experimental group, Pa O2 goes down dramatically(P<0.01), to the lowest point at the 6h, and up slightly from the 12 th to the 24 th hour; for the other group, remain stable. This shows that the value of Pa O2 reflects the severity of lung injure.5. The W/D changes and its relation to lung injury is that the W/D value for the other group remains stable, the W/D value for the experimental group raises, and up to the highest point at the 6th hour, then down slightly from 12 th to 24 th hour.According to the relation between the W/D and lung injury, the more serious the lung injure is, the higher the number goes.6. Changes of the protein content in BALF and its relation to lung injury: the content concerning the experimental group rises highly and up to the highest point at the 6h, then down slightly from 12 th to 24 th hour while the content about the other group remains stable. According to the relation, the more serious the lung injureis, the higher the content goes.7. Changes in H2 S content and CSE activity and the relation between them and lung injury:For the experimental group, the changes go down continuously at 3rd, 6th, 12 th, and 24 th hour. For the other group, remain stable. According to the relation, with the lung injure becoming serious the changes go high.8. Changes in protein expression of NF-κBp65: changes concerning the two groups go high. For the experimental group, the content of NF-κBp65 goes high with time, to the highest point at 6th hour, and down from 12 th to 24 th hour--- still much higher than the content in the control group.9. Changes in ICAM-1m RNA of the lung tissues: changes concerning the two groups go high. In comparison with the control group,the changes of ARDS. in the experimental go much higher, to the highest point at 6th hour, down since 12 th hour, still much higher than the normal level at 24 th hour.Conclusion:1. With the onset of ARDS caused by DDVP, the H2S/CSE system decreases, which revealed the system’s potential involvement in the onset.2. With the onset of ARDS caused by DDVP, NF-κBp65 and ICAM-1 increase, revealing their potential involvement in the onset. |
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