Screening Differentially Expressed Genes Related To DDVP Poisoning From Rat's Hippocampus Using Oligonucleotide Microarray

Organophosphate compounds are widely utilized as pesticide all around world, especially in the developing countries. Some people use them to commit suicide. They are also chemical weapons. In China, there are about 120,000 cases per year pesticide poisoning, of which 80% are acute organophosphate poisoning (AOPP). The mortality rate is over 30% in some parts of China, most are caused by dichlorphos (DDVP). (Don't forget to cite the reference number here) Currently, clinicians treat AOPP mainly with atropine and pralidoxime following the standard protocol. But for the CNS symptoms such as unconsciousness, convulsions, etc, there are not many medications available. Current understandings also have not provided enough explanations to the CNS symptoms. To investigate the molecular mechanism of CNS symptoms from DDVP poisoning, and to search for the functional genes related to DDVP-induced brain damage, we screen the relevant genes using the technology of oligonucleotide microarray.We composed probes of 40 genes of our interest. The probes were retrotranscribed on plata glass and oligonucleotide microarray was formed. 0. 5ml DDVP was given to the rats in experimental group and 0. 5ml-pumping brine was given to the rats in control group by hypodermic injection. Twenty minutes after convulsion, all hippocampus were collected for total RNA extraction. cDNAs were marked with Cy3 and Cy5 for control group and experiment group respectively, and hybridized with oligonucleotide...