Medical Expression Of The Hypoxia-inducible Factor-la Of The Damaged Surrounding Brain Tissue In The Experiment Of Blood Clot Removing And Brain Essence Filling Method In The Curing Of The ICH Rats Lesions

Purpose: The purpose of this experiment is to investigate the effect of intracerebralhemorrhage lesions by using of traditional Chinese medicine treatment under the guidance ofthe blood clot removing and brain essence filling method of hypoxia-inducible factor-1α. Thisessay shall discuss whether the blood clot removing and brain essence filling method canincrease positive expression of hypoxia-inducible factor-1α from ICH damaged surroundingbrain tissue, make evidence for the efficacy of traditional Chinese medicine in hemorrhagicstroke by the modern laboratory testing methods, while the accumulation of the authenticexperimental science information to further improvement and development of traditionalChinese medical science “Brains theory”.Methods: Select clean and healthy,Wistar rats, weight between240g~260g.CreateChinese medicinal formulae for hemorrhagic stroke by the principle of random whichreceived drug treatment. Create simulate operation group, ICH model group and theControlled drugs group.Guarantee25rats in each group.Sham group rat head use stereotacticpositioning technology, using50μl saline injected into the brain and in the brains6.0mmneedle retention20min,then slowly pull out the needle. The remaining three group rats headuse stereotactic positioning, taking blood from their own tail artery and then injected into thebrain to create the ICH Rats. Target at the bleeding lesions to the rat brain injected into thearterial blood from the tail of50μl and in the brains6.0mm needle retention20min,thenslowly pull out the needle. Give the experimental animals normal feeding at the sameenvironment. Take brain tissue after formalin fixation in time five days. Optical microscopyanalysis expression of hypoxia-inducible factor-1α of the surrounding brain tissue in ICH Ratslesions.Results: The Chinese medicinal formulae for hemorrhagic stroke which formulated bythe blood clot removing and brain essence filling method can effectively reduce the hematomaarea of the ICH Rats, which has better results than the ICH Rats group. It prompts that theChinese medicinal formulae played a different degree of improvement for the ICH lesionsenvironment. P<0.05.Conclusion: According to the " blood clot removing and brain essence filling method" sent hemorrhagic stroke prescription medication guide, may be the expression of HIF-lαincrease to improve the local blood supply and reduce hypoxia, inhibiting cell apoptosis,which affected brain tissue loss, help brain tissue repair.