The Research On Effects Of Removing Blood Stasis And Replenishing Essence And Marrow Therapy On Serum S100B Protein Of Experimental Intracerebral Hemorrhage In Rats

Purpose: Based on the study of neurological recovery, hematoma and serum S100Bprotein levels of experimental intracerebral hemorrhage in rats,the experiment discisses themechanism of “removing blood stasis and replenishing essence and marrow”of the treatmentof ICH,and provides a reliable experim-ental science basis for clinical therapy.Method: Healthy adult Wistar rats,male and female,weighing250g～300g or so,a totalof126.The rats are randomly divided into six groups:normal group,sham operation group,ICHmodel group,high、medium and low dose treatment group.Normal group has six rats,theremaining five group have24rats,which were randomly divided into four time points,such as1d,3d,7d,14d of postoperation of ICH,and it has6rats in each group at each time point.Weuse autologous blood injection method to make an intracerebral hemorrhage model inrats.Rats of the normal group are freely allowed to eating and drinking water.Rats of shamcontrol operation group and model group were poured1ml physiological saline into stomachper100g every day.Rats of low-dose group were poured0.5ml decoction into stomach per100g every day,rats of medium-dose and high-dose group were given2and4times the doseof low-dose group.We observe neurological deficit score of rats in each group,and extractabdominal aortic blood about4ml at the corresponding time points,centrifuge,extractserum,determine concentration of S100protein.After extracting blood,we perfuse brain troughthe heart,take out the brain,and calculate the hematoma volume with the pathological imageanalysis software Image-Pro Plus after taking pictures with the camera.Result: Making the intracerebral hemorrhage model, high, medium and low doses of“removing blood stasis and replenishing essence and marrow”of neurological deficit score,the percentage of the hematoma area and serum S100B protein levels were significantly lowerthan model group (P<0.05). And high dose group was significantly decreased than themedium and low dose group (P<0.05). The trend of serum S100B protein levels andneurological deficit scores is consistent. Traditional Chinese medicine of “removing bloodstasis and replenishing essence and marrow”can reduce the degree of neurological deficit, andpromote the absorption of hematoma and reduce the concentration of serum S100B protein. Conclusion: Traditional Chinese medicine of“removing blood stasis and replenishingessence and marrow” can improve degree of neurological deficits,promote the absorption ofhematoma and reduce the concentration of serum S100B protein.It has a role to protect thenerve tissue.Serum S100B protein levels and degree of neurological deficits have correlationafter intracerebral hemorrhage. High dose group of“removing blood stasis and replenishingessence and marrow"have the most obvious effect.