Impact Of Bai Xiangdan Capsules On Hippocampal Gene Expression Profile Of Resident Intruder Paradigm-induced PMDD Liver-qi Invasion Rat Model

Objective: To explore the targets, signal regulatory networks and mechanisms ofBai xiangdan(BXD) capsule regulate PMDD liver-qi invasion in the overall genelevel.Methods: The PMDD liver-qi invasion rat model were prepared using residentintruder paradigm. Selected the rats belong to lowest30%scores as the control andthe rats belong to highest30%scores were divided into model group, BXDadministration group and fluoxetine administration group. Analyzed gene expressionprofiles of hippocampus in each group and verified differentially expressed genesincluding Htr2c、Cdh3、Serpinb1a、Ace、Trpv4、Cacna1a、Mapk13、Mapk8、Cyp2c13、Htr1a by Q-PCR.Results: The PMDD liver-qi invasion rat models were induced by resident intruderparadigm successfully. The results of open-field test and aggressive behavior testshowed that the modeling method was reliable and stable. The aggressive behavior ofmodel rats got a certain degree of improvement after administration. The genechipdetection data indicated that the differential expression gene number of model ratswere higher than control ones and administration ones had a significantly decreaseddifferential expression gene number compared with the model rats. The mainintervention targets and mainstream signal pathway of BXD were clear. The effective targets of BXD capsules included5-Htr1a,5-Htr2c,5-Htr3a, Mapk8, Mapk13,Cacna1a, Cacn2d3, Cacn1i, Drd2, Glul and Gabarapl2. These targets improved thedisharmony state of Neuro-endocrino-immune networks by participate in Neuroactiveligand-receptor interaction, MAPK, Calcium, GABA, etc signaling regulated pathway.The results of Q-PCR tests were in accordance with gene chips data. Through thetarget genes and signaling pathways network analysis, we have preliminary elaboratedthe impact and likely mechanism of BXD treating PMDD liver-qi invasion.Conclusion: Using the resident intruder paradigm to induce PMDD liver-qiinvasion rat model was obvious and reliable. BXD capsules regulated target genes5-Htr1a,5-Htr2c,5-Htr3a, Mapk8,Mapk13, Cacna1a,Cacn2d3,Cacn1i,Drd2, Glul andGabarapl2which improved the typical symptoms of PMDD liver-qi invasion throughparticipating in neuroactive ligand-receptor interaction, MAPK, Calcium, GABA, etcsignal transduction. The mechanism required further in-depth exploration.