A Preliminary Study Of Long Noncoding RNAs(lncRNAs) In Peripheral Blood Cells On The Diagnosis Of Sepsis

Objective: Sepsis is among the most common causes of death in critical patients.Sepsis mainly depends on clinical diagnosis，but the early stage of the sepsis lack ofcharacteristic clinical manifestations. And there is currently no biomarker availablewhich alone allows a rapid and reliable diagnosis of sepsis. All of these contribute to thehigh mortality of sepsis. LncRNAs are transcripts longer than200nt that lack functionalopen reading frames. The goal of the study was to evaluate the diagnosis value of longnoncoding RNAs on sepsis.Methods: Differentially expressed lncRNAs between3sepsis patients and3healthy volunteers were screened by lncRNA microarray, then validated by quantitativerealtime reverse transcriptase polymerase chain reaction. Afterwards we involved22sepsis patients and22healthy volunteers to expand the sample size of qRT-PCR. Finallywe analyze the diagnosis value of differentially expressed lncRNAs on sepsis.Results:1. LncRNA microarray screened28significantly differentially expressedlncRNAs （fold-change≥20）, included14up-regulated and14down-regulated lncRNAs.2. QRT-PCR validated5lncRNAs: LncRNA ENST00000452391.1、uc001vji.1、uc021zxw.1、ENST00000504301.1and TCONS₀0028547. The consequences areconsistent with microarray results.3. QRT-PCR validated these lncRNAs in22sepsispatients and22healthy volunteers: LncRNA ENST00000452391.1expression level inpatients with sepsis is apparently higher than that in healthy volunteers （p<0.01）.LncRNA uc001vji.1and lncRNA uc021zxw.1expression levels in patients with sepsisare apparently lower than those in healthy volunteers （p<0.01）. They are all consistentwith microarray results. LncRNA ENST00000504301.1and lncRNATCONS₀0028547expression levels have no significant differences between sepsispatients and healthy volunteers, p values are0.213and0.064.4. The patients with sepsis can be divided into survival group, included16patients, and death group,included6patients. LncRNA ENST00000504301.1expression level, lncRNATCONS₀0028547expression level and WBC in survival ones are apparently higherthan those in the dead （p<0.05）. LncRNA ENST00000452391.1expression level, SOFAScore and APACHE â…¡ Points of survival group are apparently lower than those ofdeath group （p<0.05）. The concentration of lncRNA uc001vji.1, lncRNA uc021zxw.1,IL-6, CRP and PCT has no significant differences between the survival group and thedeath group, p values are0.914,0.203,0.559,0.914,0.449.5. LncRNA uc021zxw.1gene overlaps HLA-DRB1gene. LncRNA TCONS₀0028547gene adjoins PI3gene.HLA-DRB1and PI3are both closely related to immune response. Pearson correlationanalysis reminds us that the correlation coefficient between HLA-DRB1and lncRNAuc021zxw.1is0.9974. That means HLA-DRB1has strong correlation with lncRNAuc021zxw.1. Therefor we can infer that lncRNAs may participate in the developmentprocess of sepsis via regulating these genes’ expression.Conclusions:1. The expression levels of lncRNA ENST00000452391.1,uc001vji.1and uc021zxw.1have significant differences between sepsis patients andhealthy volunteers. So they probably can be used in the diagnosis of sepsis.2. Theexpression levels of lncRNA ENST00000504301.1, ENST00000452391.1andTCONS₀0028547have significant differences between the survival group and thedeath group. They may be associated with the prognosis of sepsis.3. LncRNAuc021zxw.1and lncRNA TCONS₀0028547may participate in the development processof sepsis via regulating the expression of HLA-DRB1gene and PI3gene.