Study On Quality Standard System And Variation Of Radix Isatidis

Radix is a commonly used Chinese medicine, is the dried root of Isatis indigotica Fort of Cruciferae, broad clinical application, except for Radix Isatidis injection liquid, the main and other traditional Chinese medicine compound preparation composed are widely used in the treatment of various diseases, with Qingrejiedu, Liangxue Xiaoban, Liyan analgesic effect, can be used for the treatment of epidemic fever, mumps, dysentery have a fever, hair spot, wind heat cold, throat swelling and decay, is universally recognized with antiviral effect of Chinese medicine is one of the few."China Pharmacopoeia" in2010edition of a pair of Radix Isatidis by microscopic identification, TLC identification, inspection,(R, S)-epigoitrin (add) content determination for quality control. Radix Isatidis is Gansu Province medicine with medicine, but the indicators are still not fully its quality was fully reflected, therefore this paper studied to improve the quality of t Radix isatidis.1Determination of1relevant chemical index component content:Is established by using the detection method of multi components, multi index:(1) simultaneous determination of cinnamic acid, salicylic acid, syringic acid, benzoic acid and Coffee acid content by HPCE-DAD method. Because the content belongs to trace, not for practical quantitative analysis.(2) in the application of HPLC method for the determination of guanosine and adenosine, uridine, component.(3) the methods used in this experiment is simple, exact method for the determination of total organic acids; Determination of total organic acids content can be used as the quality control method of Radix isatidis.2The application of chemical pattern for the quality of analysis:based on three content determination, combined with the Pharmacopoeia2010edition in Radix Isatidis (R, S)-report according to the determination of content of SPSS19.0on the spring, the use of Banlangen samples analyzed by principal component analysis. Using principal component analysis to sort the sample, results and Radix Isatidis by traditional evaluation results some correlation.3Investigation of heavy metal residues:the13batches of samples (lead, arsenic, cadmium, mercury, antimony, copper, nickel and cadmium) eight harmful elements of lead, arsenic, cadmium, copper, mercury and positive detection rate were50%,90%,100%,100%and80%, the detection rate is high; the3batch of exceed the standard CD mercury exceed the standard, the1batch,3batches of samples, exceed the standard rate of23%. Environmental impact and processing plant can not be ignored,4The kinds of64pesticide residues at the end of the investigation:19batches of samples,64residual pesticides results,(1) in19batches of samples were all detected pesticide residues, the detection rate was100%,(2) statistics has6samples exceed the standard, exceed the standard rate of31.6%.(3) the number of pesticide residues in the sample residue difference, ranged from1to3different pesticides.(4) in a sample of acetyl ammonia phosphate, the rest were poisoned bismuth methyl, Cypermethrin, fenvalerate, benzonitrile Jia Shuangling for pesticides are allowed to use.5Research of organochlorine residues:The2010edition of "Pharmacopoeia" Chinese method,15batches of samples were measured, results:the3batch of exceed the standard, but look at the Gansu plant from the content of Radix Isatidis is far lower than the relevant provisions China pharmacopoeias, explain its security is higher than other regions.6Determination of sulfur dioxide residues:select "China Pharmacopoeia2010edition of method of detection for the city of Gansu sold or origin collected14samples of sulfur dioxide pollution, the residual state. Some experimental data were provided for the future development of limit standard.7Aspergillus flavus toxin residues determination:according to the "Chinese Pharmacopoeia2010edition of method of7batches of Radix Isatidis on aflatoxin B1, aflatoxin B2, aflatoxin G1, aflatoxin G2residues, the results were not detected.8Research on genetic variation of Radix Isatidis groups of isatis root traits:Morphological shape of Radix Isatidis Gansu Province eight main distribution area of leaf and root were determined, to measure the microstructure characteristics, analysis of genetic variation in different habitats of Radix Isatidis groups of isatis root traits, variation, and provide the basis for the root of population improvement, improve the utilization value.