Study On Extraction、purification And Quality Standard Research Of Prenylated Flavonoids Of Cortex Mori And Discussing On Lowering Blood Sugar And Blood Lipid

Mulberry bark is the dry root bark of Morus alba Morus alba L., with benefits of purging lung and relieving asthma and water swelling. it is commonly used as treat the lung heat cough, edema puffiness oliguria, edema, face skin treatment. Modern research has shown, white mulberry root bark extraction has some fuctions, such as antioxidant, hypoglycemic, diuretic and inhibition of protein sugar intensification. The flavonoids of cortex:mori is the main active ingredient. Morus flavonoids in plants are connected to a prenylated, current studies show that, compounds with isopentenyl structure showed strong antitumor effect, hypolipidemic, hypoglycemic effect. In1980, Japanese scholar discovered a novel compound fat soluble components from Mulberry (Morus alba L.), namely Diels-Alder adduct.The Diels-Alder adduct is composed of chalcone and its derivatives and compounds with phenyl [4+2] cycloaddition products obtained from the reaction of addition products, all of them has still own prenylated flavonoids structure, the fat soluble part has been isolated and gotten some prenylated flavonoids and stilbenes. In addition, the water soluble fraction which has been isolated from Morus alba has polyhydroxy alkaloids and they have obvious hypoglycemic activity, the best activity of polyhydroxylated alkaloids is1-deoxynojirimycin. Research shows that, the prenylated flavonoids have many biological activities, such as cytotoxic activity, antiplatelet activity, inhibitory activity of peanut four acid5-cyclooxygenase, lipoxygenase activity, cathepsin K and5a-reductase, and anti-inflammatory, anti malaria, anti bacteria, anti lipid peroxidation and hepatoprotective activities. Diels-Alder type adducts have anti hypertension, anti microbial, antitumor, antiviral, lowering blood sugar, inhibit platelet aggregation and antioxidant The research group has been committed to the mulberry bark active ingredient, has already isolated the water soluble part and a fat soluble effective part from Mulberry (Morus alba L.). Through pharmacological experiments confirmed that, these two parts have obvious hypoglycemic activity, fat soluble effective parts for Diels-Alder type a mixture of adducts, prenylated flavonoids and stilbenes three components. The purpose of the study was isolated and enriched Diels-Alder adducts and prenylated flavonoids effective parts from fat soluble part of the white mulberry, and by pharmacological screening mulberry hypoglycemic lipid-lowering effect site, provide some data for the development of hypoglycemic.Part I:Literature reviewConcludes and summarizes the research progress of effective components, pharmacological action of mulberry bark in domestic and foreign, and pathogenic mechanism and the treatment of type2diabetes.Part II:Experimental studies1. Determination of prenylated flavonoids and Diels-Alder adduct content in root bark of white mulberry mulberryUsing UV and HPLC determination, in cortexmori and sanggenon D as the index components, determination of prenylated flavonoids and Diels-Alder adducts of mulberry bark extract.2The studies of extraction and purification process of prenylated flavonoids and Diels-Alder adducts in mulberry barkThe previous basis is using the synchronous separation of effective parts of Cortex Mori flavonoids resin exchange high macroporous resin and ion series. In this study, reflux extraction is useful as further separation and purification of prenylated flavonoids and Diels-Alder adducts by solvent method. Through the investigation of various factors, we get the optimum purification:in the experiment, wu can use30BV volume of chloroform reflux extraction of prenylated flavonoids in heating, the insoluble is extracted by filtration in ether further, the filtrate vacuum recovery to paste, and vacuum drying. The effective part of purity is more than50%.3. Quality standard of effective partsThe purpose of this research is to extract and separate mulberry bark of prenylated flavonoids and Diels-Alder adducts, exploration method and to establish the quality standard of the UV and HPLC content. 4. Effects of total flavonoids was alone, and was combined total alkaloids induced STZ diabetic mouse model of hyperlipidemia;The high fat induced by the combination of STZ replication model of type2diabetes, preliminary study of mulberry bark used alone, or in combination with alkaloid hypoglycemic effect on type2diabetes in mice.5prenylated flavonoids and Diels-Alder adducts in high fat induced STZ diabetic mouse model.The high fat induced by the combination of STZ replication model of type2diabetes, preliminary study of total flavone, mulberry root bark of prenylated flavonoids and Diels-Alder adduct hypoglycemic lipid-lowering effect.