Clonality Analysis Of Kaposi Sarcoma Leisions By HUMARA Gene

Objective: Kaposi sarcoma is also known as multiple idiopathic hemorrhagic sarcoma, it is arare multicentric tumor. Because in the naming of vascular tumours defined Kaposi sarcoma as aintermediate malignancy, and clinically often visible to the classic-Kaposi sarcoma patients hadlong-term survival with tumor, iatrogenic-Kaposi sarcoma patients’ lesions can remove afterwithdrawal of immunosuppressive agents, it has been argued to be a benign proliferation disease.To analyze the clonality in Kaposi sarcoma by evaluating X-chromosome inactivation pattern inthe human androgen receptor (HUMARA) gene analysis.Methods： Twenty-five paraffin-embedded tissue specimens were collected from female patientswith Kaposi sarcoma (n=15) or cutaneous hemangioma (n=10). DNA was extracted from thesespecimens, and digested with the methylation-sensitive restriction endonuclease HpaII. PCR wasperformed to amplify the HUMARA gene, and the amplicons were separated on10%denaturingpolyacrylamied gels and stained with ethidium bromid (EB). The loss of heterozygosity of theHUNARA gene was defined as the presence of two DNA fragments before and one fragment afterthe endonuclease digestion. The clonality in Kaposi sarcoma lesions was assessed bassed on theabrove results.Results： Among the15patients with Kaposi sarcoma,13(86.7%) were heterozygous for theHUMARA gene, of which,92.31%(12/13) showed loss of heterozygosity of the HUMARA geneon X-chromosome, suggesting a monoclonal orign. Of the10patients with cutaneoushemangioma,9were heterozygous for the HUMARA gene, and only one lost heterozygosity ofthe HUMARA gene. The heterozygosity rate for HUMARA gene was significantly differentbetween the patients with Kaposi sarcoma and cutaneous hemangioma (P<0.01). No statisticaldifference was observed in the clonality status of Kaposi sarcoma between patients of differentnationality, at different stages, or between patients with or without complicated HIV and HHV-8infection (all P>0.05).Conclusion：Kaposi sarcoma maybe is a kind of monoclonal origin tumor, and maybe there is nosignificant associations between clonality and tumor nations, staging, HIV and HHV-8infection．...