The Exploration On Significance Of Detecting The Hepatitis B Virus Large Proteins Among The People Received Physical Examination

1BackgroundThere are two billion people infected with hepatitis B virus in the world. At present, there are at least two hundred and eighty million of patients with chronic hepatitis B virus infection in the world. Seventy-five percent of chronic HBV carriers are in the Southeast Asia and sub Saharan Africa. Among these, one hundred and fifty million of patients are in china, containing two million chronic hepatitis B virus patients. And about five hundred thousand patients succumb to this per year. About108-200million people are died of persistent hepatitis B virus infection directly in the world. China is a highly prevalent area of hepatitis B virus, and which becomes a very important public health problem, so detection of hepatitis B virus has certain provisions in the entrance examination for students, the civil service, conscription and restaurant industry people. The problem pressing for solution is How to reflect the replication level of hepatitis B virus truely and rapidly in physical examination has become urgent problem to be solved. Detecting of hepatitis B virus DNA load method was adopted mainly to evaluate the hepatitis B virus replication in HBeAg negative patients, but troublesome operation, large cost and higher experimental requirements make it to be difficult to popularize and apply widely.2ObjectiveWe want to detect the leval of hepatitis B virus large proteins(HBV-LP), HBV DNA load and HBV-M to investigate the significance of HBV-LP in different physical examination in Catering practitioners, students and soldiers etc. This method might provide a new detection method for hepatitis B virus in public medical field.3MethodsEnzymes linked immunosorbent assay(ELISA) was used to detect hepatitis B virus large proteins, and the reagent were provided by Beijing Rejing biotech company, the value of cut-off is0.105. ELISA was also used to detect HBV-M serum markers, and the reagent were provided by Shanghai Kehua biotech company. Real-time PCR was applied to detect quantity of HBV DNA, and the reagent were provided by Guangzhou Da’an biotech company, we take1×103/ml increasing level of hepatitis B virus DNA copy number as positive standard, The chemical method was applied to detect glutamic-pyruvic transaminase, and the reagent were provided by Shanghai Shenneng biotech company. All of the above were operated according to the instructions strictly, and all reagent were used in validity duration. Statistical analysis was performed using SPSS12.0software. The measurement data was expressed by X±s x,2-test was used to compare the positive rate of HBV-LP and HBV DNA. p<0.05was considered as statistically significant,The linear correlation analysis was appled to measear HBV-LP S/OD values and the copy of HBV DNA correlation.4Main results 4.1There is no statistical significance between the HBV-LP method and HBV DNA method in detected709samples, The values of x2and P were respectively1.43and0.23.4.2There is also no statistical significance between the HBV-LP method and HBV DNA method in detected different models of HBV infection patients, The values of x2and P were respectively2.67,0.60,0.00and0.10,0.44,1.00.4.3S/OD of HBV-LP and the copies of HBV-DNA had positive relationship (r=0.959, P<0.05)5ConclusionsHBV-LP could reflect the level of hepatitis B virus replication effectly. HBV-LP method may be worthy using widely in physical examination of the Catering practitioners, students and soldiers etc.