Study On Oncolytic Poxvirus Carrying Lectin Genes SPL/PPA For Cancer Therapy

Nowadays cancer is still a major killer of human health, however, the traditionalmeans of cancer treatment’s effect is not ideal. With human immunology, molecularbiology, cell biology and genetic engineering technology development in recent years,a new treatment mode tumor biotherapy has already emerged. This therapy whichhas high efficiency, no radiotherapy and chemotherapy side effects can kill tumorspecifically,stimulate systemic anticancer effects and is valid for multiple lesions ormetastatic malignant.It can promote the body to quickly restore the immune systemwhich has been destoried by radiotherapy and chemotherapy, improve long-termcancer-fighting ability, and has good anti-cancer effect in the long run.Targeting Gene-Virotherapyof Cancer, a novelstrategy for cancer treatment isput forward by Pro. Liu in2001. Oncolytic vaccinia virus which is safe, simple tobuild not noly has high expression efficiency but also a natural ability to target tumortropism and lyse tumor cells. Vaccinia virus transformed can be selectively grown inthe cancer cells, so it can be used as a good therapeutic carrier. In the last few years,our research group studied the induction of cell death in cell of Pinellia pedatisectaagglutinin (PPA) gene by intracellular expression, the results of which imply thatthere is a variety of gene for cancer treatment in lectin superfamily. On the basis ofthis study, we selecte marine organisms Uiva pertusa of N-acetylglucosaminelectin UPL1, Strongylocentrotus purpuratus lectin (SPL) and lectin Pinelliapedatisecta agglutinin (PPA), studying these three lectin genes on the cellular level.In this study, the UPL1, FLAG-SPL, FLAG-PPA gene are inserted into theeukaryotic expression vector pCB. As a result of homologous recombination, thetarget gene can be inserted into TK gene of oncolytic poxvirus vectors; we can getOncoPox-FLAG-SPL, OncoPox-FLAG-PPA by screening finally. Human hepatomacell lines, breast cancer cell lines, human lung cancer cell lines, malignant glioma celllines and leukemia cell lines are infected with different multiplicity of infection of thevirus.And we detect their capability of killing tumor cells by MTT assay. The resultsshow that compared with the control OncoPox-pCB, the objective virusOncoPox-FLAG-SPL has strong destruction for different cancer cell lines and the destruction of different tumor cell lines of OncoPox-FLAG-SPL andOncoPox-FLAG-PPA is major in72h later.