Application Of Collagen Scaffold Combined With Bone Marrow Mesenchymal Stem Cells To Reconstruction Canine Bladder

Objective: In recent years, with the progress and development in the field of tissueengineering, the technology has been used in some clinical success, as the field applicationin the urinary system has become a hot spot in research and exploration. The clinicalcommon to the congenital disease,trauma, inflammation, infection, tumor and otherfactorslead to serious damage to the bladder structure and function, the need for repair andreconstruction. At present the main clinical take gastrointestinal segments as thebladderrepair and reconstruction of tissue and organsubstitutes, but because the source and functionare different, there are many difficult to avoid complications,seriously affect the patient’squality of life.The previous studies on repair and reconstruction of tissue engineering bladder show,the simple application ofscaffold materials for repair and reconstruction of bladder,such asthe existence of scaffold contracture, scar formation, serious inflammatory reaction andgraft atrophy and calcification and other complications and bladder smooth muscle layer ofregeneration ability is weak, which affects the recovery of structural and functionalreconstruction of organs. Bone marrow mesenchymal stem cells as a strong seed celldifferentiation potential, and witheasy access, passaged autologous immune rejectionability,low advantage, has become a new focus of research in the field of tissue engineering inrecent years.Part1：By using a collagen scaffold materials combined with bone marrowmesenchymal stem cells to establish bladder defectreconstruction in canine modelObjective: Use of collagen scaffold reconstruction and repair of canine bladder, so asto establish the model of canine bladdertissue engineering reconstruction feasible.Method: The12male beagle dogs were randomly divided into A, B, C three groups, A group (n=4): sham operation group, B group (n=4): collagen scaffold repair group, Cgroup (n=4): collagen scaffold combined with bone marrow mesenchymal stem cells torepair groups. Establish B, C group Beagles bladder defect animal models.For caninebladder resection, the upper bladder accounted for the total volume of2/3in resectedbladder, bladdertrigone and bladder neck, respectively using pure collagen scaffold orcombined with bone marrow mesenchymal stem cells to repair the defect of collagenscaffold of bladder reconstruction, postoperative recovery of canine incision,urine and wereclosely observed survival.Results: The experimental group animals except for one in2days after opration wasdead for infection of urinary extravasation as a result of bladder rupture,else were normalliving, removal of catheter to urinate normally.Conclusion: The tissue engineering materials combined with bone marrowmesenchymal stem cells in bladder reconstructionis feasible. Part2： Experimental study collagen scaffold combined with bone marrowmesenchymal stem cells after reconstruction of canine bladderObjective:Through the use of collagen biomaterials with bone marrow mesenchymalstem cells to repair and reconstruction of canine bladder.Method:The12male beagle dogs were randomly divided into three groups, group A(n=4): control group, B group (n=4): pure collagen biomaterials repair group, C group(n=4): collagen bio materials combined with bone marrow mesenchymal stem cells torepair group, the establishment of B and C group of canine bladder defect animal model,respectivelyuse pure collagen biomaterials or collagen materials combined with bonemarrow mesenchymal stem cells inbladder reconstruction. In each group23weeks afterrepairurodynamic examination and urinary tract imaging, both in the animal were sacrificedafter24weeks, the samples were taken, and through to the gross observation andhistological examination to evaluate bladder bladder regeneration.Results: Postoperative in B group an animal for bladder rupture of abdominal infectiondied2days after stenting, the restwere normal in life, can be normal voiding after removal of catheter. On the macroscopic observation showed thatscaffold biomaterials are degradedand absorbed, bladdernot found occupying lesions, stone. The urodynamic data,statisticallyconfirmed that A, in group C, maximum bladder capacity, maximum bladder compliance,detrusor pressurehad no significant difference (P>0.05), and B group in the maximumbladder capacity, bladder compliance, the difference was statistically significant (P<0.05).Imaginggroup had no obvious abnormalities. The histological examination showed that allbladder transitional epithelial cell regeneration were satisfactory, but the B group bladdersmooth muscle regeneration was significantly poorer, no complete normal muscle tissueregeneration, in which Cgroup of bladder smooth muscle layer is the layer ofsmooth muscleregeneration, complete.Conclusion:Through biological scaffold materials of tissue engineeringseed cellscombined with the reconstruction of bladder,confirmed using collagen scaffolds combinedwith bone marrow mesenchymal stem cells can promote the bladdertissue regeneration andrepair ability better, especially inbladder smooth muscle regeneration. Part3：To investigate the effect of upper urinary tract structure and function aftercollagen scaffold reconstruction of canine bladderObjective：Application of collagen scaffold to reconstruct the canine bladder, andevaluate influence of the structure and function on the upper urinary tract.Methods：The8male beagle dogs were randomly divided into two groups,group A(n=4): control group, B group (n=4): collagenrepair group, the B group of beagle dogs toestablish bladder defect model, application of collagen materials forrepair andreconstruction of the bladder defect. In each group23weeks before and after the restorationof renalultrasonography and blood flow imaging, biochemical index of renal function andurine volume were24h,24h urinary protein quantitative determination, and the animal weresacrificed24weeks after operation, the specimens were taken from the kidney, ureter,general observation and histological examination, through the influence of theobservationand analysis of index of bladder reconstructionin upper urinary tract structure and function. Results：Postoperative in B group an animal for bladder rupture of abdominalinfection died2days after stenting, the restwere normal in life, can be normal voiding afterremoval of catheter. Through the determination of biochemical index,renal function beforeand after operation of24h urine and24h urinary protein quantitative results werestatistically analyzed, there was no significant difference of the aboveindexes between thetwo groups (P>0.05). Renalultrasonography and blood flow imaging showed that allanimalkidney morphology, structure, renal arteriovenousblood flow signals were no exception, nota kidney stone,renal pelvis seeper, expansion of renal cystic space occupying lesion.Through the observation of thespecimens showed that all animal kidney, ureter stone,foundno tumor formation, no obvious space occupying and new biological change. Histologicalobservation found among groups mucosal cell layer integrity, epithelial cells arranged inneat and compact, ureteral and renal interstitialhyperemia and edema was not found and theinfiltration of inflammatory cells, glomerular, tubular noclear pathological changes, renalmesangial cells without proliferation.Conclusion： Application of collagen scaffold reconstruction of canine bladder not onupper urinary tract adverse effects,reconstruction of bladder tissue engineering applicationvalue of this method is further.