Study On The Differences Of Heparanase Gene Expression And Cell Adhesion In Human Hepatocellular Carcinoma

Objective： To explore the expression of human heparanase in hepatoma cells, andexplore the impact of HPSE on platelet adhesion and aggregation activating byhepatoma cell.Methods: selecting human liver cells LO-2, two kinds of liver cancer cells:HepG-2and MHCC97-H, using the rat blood to isolate platelets, platelet suspension wasprepared and plated collecting two hepatoma cell in the logarithmic phase,this twocells suspensions were prepared and injected into the platelets, to observe the adhesionof cancer cells;useing Real-time PCR to measuring the expression of mRNA of HPSEin the three cells.Results: Detecting the adhesion of gene expression of HPSE in MHCC97-H cells wassignificantly higher than HepG2cells by resting platelet adhesion.The expression ofmRNA of HPSE gene was detected in Lo2, MHCC97-H and HepG2cells by real-timePCR,and discovered the expression of mRNA of HPSE gene was higher inMHCC97-H than HepG-2;successfully screened HPSE gene high expression cell linesMHCC97-H and HPSE gene low expression cell lines HepG2cells.Conclusions: In hepatoma cell lines, cell adhesion rate of cell lines HPSE geneexpression in cell lines expressing HPSE low, may be associated with platelet adhesionand thrombus formation, liver metastasis, requires further study.