Atractylenolide Ⅰ Acting On MyD88+Human Ovarian Cancer Cells Have An Effect On Expression Of Indoleamine2,3-dioxygenase(IDO)and T Lymphocyte Proliferation Activity In Peripheral Blood

Objective Study the effect of atractylenolide Ⅰ acting on human ovarian cancer cells (MyD88+)expression levels of the protein,indoleamine2,3-dioxygenase (IDO). Research on human ovarian cancer cells (MyD88+) and human peripheral blood T lymphocytes were cultured atractylenolide one pair of T lymphocyte proliferation activityMethods In this study usingWestern Blot methodto test:Detection atractylenolide Ⅰ impact on human ovarian cancer cells(MyD88+) indoleamine2,3-dioxygenase (IDO) protein expression levels. Application MTT assay:detection of ovarian cancer cells(MyD88+) with healthy human peripheral blood T lymphocytes after co-culture atractylenolide one pair of T lymphocyte proliferation activityResults(1)There are a high expression of indoleamine2,3-dioxygenase’s protein in MyD88+ovarian cancer cells;(2) Atractylenolide Ⅰ of different concentrations induced MyD88+ovarian cancer cell lines24h,48h later, with the extension of the increase atractylenolide Ⅰ concentrations and duration of action of drugs inhibit IDO protein levels more obvious.(3) WesternBlot method results showed:it is no statistically significant when the drug concentration of the atractylenolide Ⅰ are between10umol/L and50umol/L acting the ovarian cancer cells24h down the multiple of IDO protein expression in the tumor cells of MyD88+ovarian cancer (P>0.05);(4)Atractylenolide Ⅰ different concentrations induced ovarian cancer cells(MyD88+), as increasing of atractylenolide Ⅰ concentration and the role of the time, people inhibition of ovarian cancer cells(MyD88+) expressing IDO protein levels in48hours, concentration of atractylenolide Ⅰ was the strongest in the100umol/L(P<0.05), there are significant differences.(5) Different concentrations (0μmol/L,10umol/L,50umol/L,100umol/L) were induced atractylenolide a different proportion of peopleovarian cancer cells (MyD88+) and normal human peripheral blood T lymphocytes after72hours of culture, along with the atractylenolide Ⅰ concentration’s increasing, the more obvious the proliferation of T lymphocytes (P <0.05).(6) In the same drug concentration of atractylenolide I,a different percentage of people ovarian cancer cells(MyD88+) and normal human peripheral blood T lymphocytes, in the ratio of S:T=8000cells/well:40,000/well atractylenolide I when the concentration of100umol/L, the most significant proliferation of T lymphocytes.Conclusions (1) There are a high expression of indoleamine2,3-dioxygenase’s protein in MyD88+ovarian cancer cells;(2) Atractylenolide I could cut the expression levels of IDO’s protein in ovarian cancer cells(MyD88+) in a time-dependent and concentration-dependent manner by TLR4/MyD88pathway inhibiting activity;(3) Atractylenolide I passed down the expression of IDO protein,which is associated with the T lymphocytes is immunosuppression molecules, avoiding the decomposition of tryptophan and accumulation of kynurenine which is the tryptophan of metabolites, promoting the proliferation of T lymphocytes with a drug concentration-dependent manner.