Intra-articular Injection Of Parecoxib Sodium Intervention Rabbit Knee Osteoarthritis Merger Synovitis

Object:To observe articular cartilage and serum levels of inflammatory cytokines by animal experimental in white rabbit model of the osteoarthritis, which explore a reasonable way of intra-articular medication safety thus provide new ideas for clinical treatment。Methods:60New Zealand white rabbits were divided into six groups:parecoxib sodium group (A), sodium hyaluronate group (B), the Hormone group (C), model group (D), the non-bone arthritis group (E group), blank control group (F)。 Each group of10rabbits of the tubular cast immobilization modeling put in the A、B、C and D。 Take10rabbits as osteoarthritis group (E) and another10rabbits as a blank control group (F), without any treatment。 Fixed in accordance with the Vandman method of experimental animals in the holder。 the white rabbit left rear knee plaster cast straight bit down to the above the ankle would be fixed to the top of the hip plaster and the ankles dorsiflexion30-40°。After the6weeks plaster external fixation to remove the animal models of osteoarthritis。 Another10as a blank control group without any treatment。 Randomly selected two rabbit models while the plaster cast were fixed2,4,6weeks,which air embolism was put to death by the ear vein row。 Taking articular cartilage pathology of the to observe rabbit models after successful removal of external fixation gypsum。After successful modeling, based on the pre-experiment and reported in the literature to determine the dosage and administration, while not treatment was gived to the blank control group。 Parecoxib sodium (300ug in0.3ml) was injected weekly into the group A and B respectively for4weeks,while the0.3ml glucocorticoid was administered to those in the group C。0.3ml normal saline was injected weekly into the group E for4weeks,while parecoxib sodium (300ug in0.3ml) was administered to the group E。At the third week and the sixth week after treatment,four white rabbits in each group were selected randomly。2ml of blood prior to sacrifice by ear vein and the Serum was allowed to stand。 Serum and synovial fluid in the-20degrees refrigerator were left to detect the concentration of Related inflammatory factors。 The disease seized specimens of synovium and cartilage of the femoral condyle parts of light microscopy。 The synovial tissue was fixed in10%neutral buffered formaldehyde solution10hours After rinsing in ice saline。 The removed cartilage tissue was fixed in10%neutral buffered formalin for24-48hours。 decalcification of specimens with the10.6%neutral EDTA and decalcification solution at46degrees incubator daily line once the decalcification fluid replacement, while the decalcified time is about10-12days。 Morphological changes further embedded in paraffin。 Polysaccharide coloring will be observed by HE and AB-PAS staining of cartilage according to OARSI score。 Acidic mucus was blue, the neutral mucus was neutral, mixed mucinous were purple and blue nucleus。 On the synovial production biopsy the parallel correlation staining according to the Mankin’s score。 Synovial fluid, blood concentration of IL-1β and other inflammatory cytokines in detecting serum was collected using the ELISA sandwich method mark。 The IL-1β, IL-6and TNF-a in the synovial fluid was a colored reaction, the enzyme calibrators460nm reading the OD value of the standard curve, while concentrations of the IL-1β, IL-6and TNF-a were calculated。Experimental result:1. Groups Knee general observation Results: Compared with the normal control group, osteoarthritis model group showed varying degrees of joint effusion, synovial hyperplasia edema。 Articular cartilage surface gloss local area decreased, articular surface under the bone was exposed。 Cartilage degeneration of Parecoxib sodium groups and the HA groups were lighter than those of the model groups。 The cartilage of the Hormone groups are serious compared with the control groups。 Synovial and cartilage lesions in the non-osteoarthritis group are similar to the control group。 synovial lesions in the Parecoxib sodium group and the hormone group are better than the model group。2.HE and AB-PAS staining:HE staining of the synovial expressed as the varying degrees of inflammatory cell infiltration in the each experimental group, in which the model group is the most serious。 smooth, clear hierarchy of cartilage cells in the control groups。 a large number of chondrocytes column, the no cluster poly cartilage cells and karyopyknosis, phenomenon tide line complete。 HE staining of cartilage performance in the Parecoxib sodium groups better than the model groups and Hormone groups。 HE staining of cartilage performance in the model groups better than the Hormone groups。 In addition to the group E and group F, in the other groups expressed as the loss of transfection and proteoglycan in the matrix with different degrees of cartilage AB-PAS。 Cartilage lesions in the non-osteoarthritis group are similar to the control group。3.Inflammatory cytokines:compared to the model group, the contents of the IL-1β, IL-6and TNF-α concentrations were significantly increased in the blood supernatant and synovial fluid of the model group(P<0.05)。 Compared to the model group, the contents of the IL-1β, IL-6and TNF-α concentrations were significantly increased in the blood supernatant and synovial fluid of the Parecoxib sodium group and hormone group (P<0.05)。 The contents of the IL-1β, IL-6and TNF-α of the blood supernatant and synovial fluid in the Parecoxib sodium group and hormone group decreased than the HA group。Conclusion:Compared with sodium hyaluronate, parecoxib sodium can significantly alleviate the symptoms of synovitis in the rabbit models of osteoarthritis。 Compared with the hormone groups, cartilage lesions of the parecoxib sodium groups seen less damage。 By the animal experiments,we confirmed that intra-articular parecoxib sodium could be used as a new treatment option in osteoarthritis patients, especially mergerred with marked synovitis。 ABSTRACT Osteoarthritis (OA) is common in the elderly, multiple and more difficult to treat a chronic osteoarthritis. Current drug treatment of knee osteoarthritis in many ways, including intra-articular injection because it is topical, but also a direct effect on arthritis, osteoarthritis treatment is the most used and most effective methods. a single medication may exist at the same time play an anti-inflammatory effects may be induced articular cartilage injury, or anti-inflammatory effects in the same time to promote articular cartilage repair is not ideal, Therefore, the best method of treatment should be a comprehensive analysis of patients with joint structure, function and possible pathogenic factor to understand the expectations of patients with joint recovery and extent of use to design an individualized treatment plan。 the paper now in recent years, intra-articular treatment of osteoarthritis drug treatment status are reviewe below.