Study On The Effect And Mechanism Of Human Osteoarthritis Of Knee Articular Cartilage Degeneration Cells Intervention Of Astragaloside

Objectives:Effect of expression of Huang Qi is effective in the composition of Astragaloside on KOA degeneration articular inflammation factor IL-1 and TGF-B 1, VEGF, type II collagen, proteoglycan gene mRNA.Methods:The degeneration of knee articular cartilage from patients with KOA after operation, cell culture, the isolated chondrocytes passaged, cells from the third passage, by hematoxylin eosin staining and type II collagen immunofluorescent staining of cartilage cell ratio, VEGF, TGF-B 1, type II collagen, proteoglycan immunofluorescence staining and observation of cartilage cells, degeneration, divided into 6 groups (group A:cartilage cells in+DMEM group; B group:the cartilage cells into the 25mmol/LAS; C group:the cartilage cells into 50mmol/LAS; D group:the cartilage cells into 75mmol/LAS; E group:the cartilage cell entry into 100mmol/LAS; F group:the cartilage cells into the 500mmol/LAS),4h,8h,24h,48h,72h the 5 time points, the degenerative cartilage were detected by real-time fluorescence quantitative polymerase chain reaction within the cartilage cells VEGF, TGF-, Co12A1, ACAN B 1 gene expression of mRNA.Results:In a certain concentration, within a certain period of time the astragaloside can improve cartilage cells TGF-B 1, VEGF, Co12A1, ACAN gene expression of mRNA, reduce the expression of cartilage cell inflammatory factor IL-1, to protect the cartilage, delay the degeneration of articular cartilage effect, discovered by toluidine blue staining and different time, different time with different concentration of degeneration cartilage cells of different concentrations of cartilage degeneration of TGF-B 1, VEGF, type II collagen, proteoglycan immunofluorescence staining, in certain concentration, within a certain period of time the situation degenerated cartilage cells of astragaloside of proliferation and aging slowed down significantly, and when the astragaloside concentration is too high, further accelerated aging the degeneration of cartilage cells.Conclusions:The series of experiments, the medicine Huang Qi mainly in the main component can effectively control KOA,, which provides theoretical basis and mechanism of astragaloside, which provides a solid theoretical foundation for the use of clinical application of Qi Teng soup.