Electrospun Nanofibers-based Chemo-photothermal Combination Therapy To Prevent Local Tumor Recurrence After Surgery

Background:The worldwide incidence and mortality of cancer were increasing.The surgery is usually the first choice for those patients with early cancer,however, as high as70%of patients receiving complete tumor resectionwill undergo tumor recurrence and distant metastasis. The localrecurrence, especially positive margin recurrence, is one of the mainreasons for the treatment failure.With the development and application ofnanoscience and nanotechnology in the biomedical field, the nanofibersprepared by electrospinning technique which had a large specific surfacearea, porosity, drug loading capacity, were widely used in the controlledrelease of antibiotics,anticancer drugs,biological macromolecules and soon.However, the studies related to the application of the drug-loadedelectrospun nanofibers in the treatment of cancers, especially in theprevention of local tumor recurrence, were still rare.Objective:To study the efficacy and safety of the nanofibers-basedchemo-photothermal combination therapy applied in the prevention oflocal cervical tumor recurrence after surgery. Methods:a.The preparation of the cisplatin and indocyanine green loadedsilica nanoparticles and the measurement of photothermal conversioneffects.b.The evaluation of cells cytotoxicity of nanoparticles-basedhyperthermia chemotherapy in HeLa cells by MTT assay.c.The preparation and characterization of silica nanoparticles-loadedelectrospun nanofibers.d.The establishment of the cervical cancer local recurrence model inmice and the application of nanofibers in local hyperthermiachemotherapy to prevent the local tumor recurrence.Result:a.The photothermal conversion efficiency of the indocyaninegreen(ICG)-loaded silica nanoparticles is better than that of free ICG.b.Silica nanoparticles-induced hyperthemia chemotherapy couldproduce the strongest cytotoxic against the cervical cancer HeLa cellscompared with hyperthermia or chemotherapy alone. The blanknanoparticles were highly biocompatible and safe.c.In the animal experiments, the electrospun nanofibers-based localhyperthermia chemotherapy could suppress postoperative local tumorrecurrence, prolong the life cycle, and reduce systemic toxicity in mice.Discussion:The electrospun nanofibers-based local hyperthermia chemotherapy used in the high-risk postoperative tumor recurrence areas, is aeasy-to-use, feasible, effective, safe, and new strategy to prevent localtumor recurrence, which has tremendous clinical poentials.