Cognitive Deficits And S100β In The Hippocampus Triggered By Burnt Trauma Are Exacerbated In Adult Rats

Objective：Delirium is an acute non-specific dysfunction existing in the substance of brain.Because of burn mechanisms of delirium have not clear, and there is no clearbiological marker for clinical diagnosis of the disease. At present, only rely oncommon mental illness in patients with clinical scale speculated that the illness, orthrough the electroencephalogram waveform judge patients delirium state. Thesemethods can’t meet the diagnostic requirements and have many defects. This paperexplore the extensive burns leading to cognitive impairment in adult rats and relationwith the level of S100β.It provides accurate, convenient and quick diagnosis andtreatment of basis to improve the patients quality of life.Method：(a) Adult large Ⅲ degree burn model in rats. Rats were randomly divided intomodel group (8), and blank control group (8), burn group (divided into burns after1d,3d,5d,7d and14d and21d in each group of8). Experimental animals can satisfythe requirements of environmental and feeding. Exclude health rats and pay for thenumber of experimental animals in each group. All rats were raised in theenvironment for7days. Modeling of Wistar rats by intraperitoneal injection of10%chloral hydrate and using depilate cream on the back skin after anesthesia effect.(asshown in figure1) Accroding to rehydration formula of Parkland, scald rats wereinjected with ringer’s lactate solution by intraperitoneal injection. The model iscomplete after liquid recovery and constant temperature blanket (38-40℃) of heatpreservation, recovery of rats independent activities.Blank control group were notburn but disposed with the same situation.(b) spatial learning, cognitive ability,Morris water maze. The device is a diameter is150cm depth of50cm round black tank (water temperature20to22℃), a black platform under the straight horizontal1.0cm. Sink and so on can be divided into four quadrants. And then put into thequadrant, free swimming in120s, or find the platform of rats, the rat experimentterminated, if within120s rats did not reach the platform, has guided the find theplatform, and stand on the platform of10s.5days after the training, the blank controlgroup and burn group (1d,3d,5d,7d and14d and21d)8rats repeated water mazeexperiment, determined the position of platform can burn group memory. Observingand recording the rat swimming speed, time of arrival in platform used and paraderoute in rats.(c) According to beta protein extraction.1, weighing.2, homogenate,3centrifuge,4vibration,5Centrifugal again.(d) according to beta protein ELISAdetermination.Results：(a)Morris training of adult rats before5days. Morris water maze map of rats was neat parade route, and succeed to step on the platform during the experiment in the120s (figure3.8).(b) Blank control group rats Morris train diagram.In1,3,5,7,14,21d, Morris water maze map of rats, rats almost marched straight to the platform, the success on a platform (as shown in figure3.9, as shown in figure3.16).(c) Burn the experimental group rats Morris train diagram.In1,3,5,7,14,21d Morris water maze map of rats, rats parade route gradually becomes complex, the march way from straight to along the walls, and by the success on the platform to the120s unfinished experiment (as shown infigure10,11,12,13,14,15).(d) Blank control group and experimental groupof rats marches parade speed, distance and time of reaching platform. UsingMorris water maze system records parade distance (see table3.1), time of platform (see chart3.2) and speed (see table3.3). Results show that rats with burns whose spatial memory and learning ability were decline. However each parade reveal that the speed of the rat are not associated with the increasing of time and distance. Parade route, when burned rats after intervention parade’s journey has obvious rise, then fell back (as shown in figure3.18,3.20). Parade time to both the maximum and the minimum and average experimental group spe nt more time than the control group s shown in figure3.19). Each group of rats parade speed is the ups and downs, having no obvious characteristics between groups (as shown in figure3.21).(f) Burn the hippocampus of rat according to beta expression. By using ELISA to detect the hippocampus of beta protein expression in table1(see table3.2). Adding more the determination of protein concentration, the rat burn time and beta protein concentration into positivecorrelation (such as table3.2in figure3.22)Conclusion：1. Large area burn caused experimental cognitive dysfunction in rats.2. Extensive burns delirium cause damaging to the central nervous system.3.S100β canbe used as burns delirium diagnostic basis in the future...