Study On The Integrated Manufacturing Technology Of Smalt-diameter Artificial Blood Vessel By Laser Fabrication

One of the most severe forms of heart disease is associated with atherosclerosis,a process that causes narrowing of the arteries. For coronary arteries, this narrowingleads to a weakening of the myocardium, and ultimately to myocardial infarction. Themost effective methods treating this disease are vascular repair, bypass, andreconstruction using the substitutes. Each year a half million such surgeries areperformed in the United States alone. Even so, there are many patients in need of suchsurgery procedure is not possible because their native vessels-either from beingdiseased themselves or because of previous surgery-are not available for use. Evenwhen appropriate native vessels are available, however, removing them from theirnormal positions in the vasculature is less than desirable. Because of the small caliberof coronary artery, it was this need for an alternative to native blood vessels that leadto one of the Holy Grail of tissue engineering, creating a small-diameter blood vesselsubstitute.The development of a tissue-engineered blood vessel substitute has motivatedmuch of the research in the area of cardiovascular tissue engineering over the past20years. Several methodologies have emerged for constructing blood vesselreplacements with biological functionality. These include cell-seeded collagen gels,cell-seeded biodegradable synthetic polymer scaffolds, cell self-assembly, andacellular techniques. however, only a few have achieved clinical success with thistechnique，This requires that the engineered substitute not only be nonthrombogenic,it also must exhibit vasoactivity and possess appropriate mechanical properties, oneswhich some believe must match those of a native vessel. Although these approacheshold much promise, the goal of developing a next-generation vascular graft will likelyrequire knowledge gained from each one.In this paper, we design a rapid method to fabricate small-caliber vascular graftcalled rotating exposure method by Optical process integration technology. First we design the square wave generating circuit adjusted by the555Timer to realize theduty ratio. The rotation rate could be adjusted by Square wave drive motor. Mixedthe biological material PEGDA and Daracure1173to complete the preparation ofprecursor solution, then pour it into a cylindrical mold, which is rotatable andtransparent. The cylindrical mold is composed of two quartz glass tube which havedifferent diameter, and the difference between one inside diameter and the otheroutside diameter determines the Thickness of the artificial blood vessel. The vascularwall has its complicated architecture and unique mechanical properties, when thepolymerization processing completed, bio-mimic micro-and nano structure wasfabricated on the inner surface using the dual-beam interference. Finally thepaper strat to a series of experiments using human umbilical vein endothelial cellsin the inner surface with micro structure of the vessel substitute to proved that thesmall caliber artificial blood vessel has good biological properties, which is expectedto be used in clinical trial via a further research.