Preparation And Biocompatibility Evaluation Of PDLLA And Nano-hydroxyapatite Fiber Membrane

Clinical rehabilitation of bone defect using tissue engineering materials tosubstitute natural bones has become a research hotspot. In this paper, porouspoly-dl-acid (PDLLA) electrospinning fiber membrane was prepared, andnano-hydroxyapatite (nHA) was adsorbed and wrapped into it during the uniqueshrinking progress of the PDLLA fiber membrane to fabricate the PDLLA/nHAcomposite membrane scaffold for bone tissue engineering. Then its properties ofthermodynamics, mechanics, biocompatibility and degradation were researched, andthe results are as follows:Firstly, porous electrospinning fiber membrane of PDLLAwas prepared. And thebest conditions (solvent and electrospinning environmental conditions liketemperature and humidity) to fabricate fibers with good morphology was confirmed.Secondly, nHAwith good morphology and crystal form was synthesized and wasabsorbed and wrapped into the PDLLA fiber membrane. The combination was probedby scanning electron microscope (SEM), infrared spectroscopy (IR) andthermogravimetric analysis (TGA). It was proved that the shrinkage degree of PDLLAfibrous membrane and the dispersion effect of nHA were both influenced by theconcentration of the ethanol-water medium. In this case, the best concentration to getproper composite membrane was confirmed. Then the morphology, thermodynamicproperty, hydrophilicity and tensile property of the composite membrane were tested.Thirdly, the biocompatibility and degradation property of the compositemembrane were researched. Chondrocytes from rabbit were inoculated in thecomposite membranes and cultured in vitro, and the results observed under SEM andfluorescent confocal focus microscopy (FCFM) showed that the chondrocytes grownquite well. The observation of the degradation progress showed that the existence ofnHAcould neutralization the acid from the decomposition of PDLLAto some extent.In this paper, PDLLA/nHA composite membrane with proper hydrophilicity, mechanical property and biocompatibility was fabricated through the shrinkage ofporous PDLLA fiber membrane. This composite membrane has potentialapplication value as scaffold material in bone tissue engineering.