Reserch Of The Impact Of Electrostatic Spinning Small Diameter Artifical Blood Vessels’s Morphological Parameters To Its Performance

With the continuous improvement of people’s living standard, physical health and the national level of medical care are more and more widely attentioned. Vascular disease is one of the most common health problems, Blood vessels is the transport corridor of body’s blood, when the blood vessels due to aging, thrombosis, or damage can’t normally work, human life is threatened. So, vascular replacement, bypass ect. surgery arises at the historic moment, every year, the world has a considerable number of vascular surgery to promote the further research of the artificial blood vessels.At present, studies of larger diameter artificial blood vessel to replace artery have been relatively mature, however, clinical application of the artificial blood vessels of less than6mm in diameter used to replace the small arteries and veins has yet to be improved. The main reason is that thrombosis and neointimal thickening appear after transplantation, cause blood clots, eventually lead to vascular transplantation failure. So, the long-term patency rate of small diameter artificial blood vessels has become the research focus now.Now, the problem to restrict artificial blood vessel development in our country is the lack of the study of artificial blood vessel’s biomechanical properties characterization, in the process of research and development, there is no proper test methods to monitor and evaluate in vitro of the quality of the products, only through short-term animal experimental to study. Most of test on the mechanical properties in vitro is static, lack the dynamic test be similar to the human environment. And simple static mechanics performance cannot completely characterize the biomechanical properties of artificial blood vessels; also need to further simulate conditions of human for dynamic test, to better evaluate the actual availability of artificial blood vessels. The reason that restenosis occurs after implantation of the small blood vessels is multifaceted, one of the important influence factors is the radial compliance of small blood vessels.Against the research situation of artificial blood vessels, this paper organically combines the electrostatic spinning forming technology, the characterization techniques to electrostatic spinning fiber, the dynamic testing technology to small vessels and cell biological evaluation technology together, The main study and thesis structure including:The first chapter mainly summarizes the artificial blood vessels, especially the research status and significant problems of small diameter artificial blood vessels, and medical requirements and characterization parameters to the properties of artificial blood vessels, at the same time; this paper introduces the research status of the electrostatic spinning small diameter artificial blood vessels.In the second chapter, we selected PET that has excellent mechanical properties and structure stability as base material, then used PET to prepare electrostatic spinning small diameter artificial blood vessels, discussed the influence of the solution and process parameters on the structure and morphology of the small blood vessels.In the third chapter, electrostatic spinning equipment was improved, from the morphological changes of receivers, prepared small blood vessels of different structure parameters, including straight, tapered, straight type corrugated and conical corrugated, as well as small blood vessels of different taper and corrugated spiral number, to further discuss the influence of small blood vessels structure change to the PET small blood vessel dynamic performance.In the fourth chapter, cell culture test of PET small blood vessels had been discussed, and the cell proliferation of PET small blood vessels before and after corrugated also be studied. The results show that:the cell proliferation effect of PET small vessels was better. Corrugation had certain influence on the cell proliferation of blood vessel wall, cell proliferation reduced after corrugated than that before corrugated, but corrugated spiral number differed, cell proliferation did not show obvious differences.In the fifth chapter, the topic was summarized and prospected.