Experimental Study Of Bone Marrow Mesenchymal Stem Cells Into Bone Transformation Induced Double Gene Vector

Purpose: This study investigated the effect of IGF-I and BMP-2in the course of therelationship between bone formation and bone formation，So that we can find a new way toimprove bone healing, repair of bone defects.Method: Construction of the IGF-1and BMP-2expression in eukaryotic cells of vectorpEGFP a N1/IGFI a BMp-2， will provide exogenous IGF-1and BMP-2cDNA importBMSCs, so normal expression to enhance BMSCs osteogenic ability.Result:A Eukaryotic expression vector and were identifiedUsing genetic engineering techniques were constructed pEGFP a Nl/IGF1, pEGFP aNl/BMP2single gene expression plasmid and pEGFP a NI/IGF1a double BMP2geneco-expression plasmid.B In the rat femur and tibia bone marrow cultured in vitro.The results can be seen under a microscope BMSC colony formation in rats and continueto grow, while80%of the cells near the confluence of the cells ALP expression, can beformed after the confluence of calcification. BMSC collection easily visible,, a lot of abilityand amplified osteogenic differentiation without significantly reduced.C And in vitro expression of IGF-1-2and osteogenic differentiation of cells transfectedwith BMPFluorescence microscopy cell transfection, calcium drilling method using a modifiedGomori count ALP (ALP)-positive rate and calcified nodules. The test results show that after48hours, the transfected cells were positive, pEGFP a1/IGF1a maximum transfectionefficiency BMp2. Expression ALP, and a calcified nodule formation.Conclusion:BMP alone has a strong osteoinductive activity of BMP-2to induce boneformation, IGF-l induction of BMP-2into enhancing the role of bones. The expressionconstruct pEGFP a Nl/IGF-1a BMP-2plasmid in eukaryotic cells to be able to smoothlyapproach cationic liposome transfected into BMSC. IGF-1, BMP-2gene modified BMSCmay be directed differentiation into osteoblasts.