Effects Of Montelukast Sodium On Experimental Abdominal Aortic Aneurysm In Rats Model

Background:Montelukast is a new generation of compounds, originally applied to thetreatment of bronchial asthma. Recent studies have shown that montelukast toreduce human abdominal aortic aneurysm specimens have a role in MMP levels,which may come into play by inhibiting CysLTs. Whether it is necessary toexplore CysLTS biological pathways leading to abdominal aortic aneurysm is animportant part of providing a reference to the pathogenesis of experimental drugtreatment of abdominal aortic aneurysms.Objective:The experiment is based on the established rat model of abdominal aorticaneurysm on the use of montelukast stomach perfusion through rat arterialdiameter, arterial opening angle and pathological changes in the arterial wall ring,to explore montelukast on rat ventral inhibition mechanism aortic aneurysmformation.Methods:Using SD rats were established by CaCl2outer membrane was incubatedAAA model, using statistical software will generate a random number ofexperimental animals were randomly assigned to surgery and treatment factors aretaking montelukast by way of persistent postoperative medication4w,montelukast treatment group and the control group given CaCl2epicardial veininvasion when laparotomy.4w observed in rats after laparotomy, measure thediameter of the abdominal aorta of mice and ring opening angle, the abdominalaorta specimens, HE staining and immunohistochemical observation of MMP-2and MMP-9distribution difference in the arterial wall. Results:22S-D rats were successfully established AAA model, after intervention bythe experimental program were obtained22parts of rat aorta specimens.(1)ultrasonic testing and the general concept of the control group, the diameter of theabdominal aorta and the largest open-loop corner, followed by montelukast group,sham group minimum.(2) HE staining showed thickening of the outer membraneof the abdominal aorta in each group, the film is widened, elastic fiber breakage,reducing the number of layers, partial rupture of the membranes, the outermembrane and the membrane inflammatory cell infiltration.(3) MMP-2andMMP-9immunohistochemical staining distribution of MMP-2and the outermembrane with the membrane-based, especially in the control group was heavy;distribution of MMP-9was mainly close to the outer membrane and themembrane side of the membrane-based, less MMP-9levels in most of the filmorganization.Conclusions:Montelukast CaCl2-induced AAA model in rats has a protective effect.In the rat model, montelukast reduced in rats with open abdominal aorticdiameter and Cape role in the occurrence and development of AAA, theindirect evidence CysLTs can damage collagen and elastin fibers.Montelukast inhibits AAA diameter caused by the CysLTs increase anddecrease in arterial wall elasticity process. CysLTs may prompt majordamage collagen and elastic fibers of the aortic wall to accelerate thedevelopment of abdominal aortic aneurysms by accelerating MMPs.