| ObjectiveTo investigate the effects of seaweed and liquorice, one pair of herbs in the18contraindications, as well as the seaweed extract on the expression of Na/taurocholate cotransporting polypeptide(Ntcp) in liver.Methods1. A total of72Kunming male mice were divided into six groups, mice were orally administered with saline, seaweed decoction, liquorice decoction, seaweed and liquorice mixed decoction at the ratio of1:1,1:3, and3:1for14days. The dosage given to mice was equal to12g/kg crude herbs. After drugs administration, Ntcp expression in the liver of mice was examined by Western blotting technique.2. A total of48Wistar male rats were divided into six groups, rats were orally administered with saline, seaweed decoction, liquorice decoction, seaweed and liquorice mixed decoction at the ratio of1:1,1:3, and3:1for14days. The dosage given to mice was equal to6g/kg crude herbs. After drugs administration, serum biochemical parameters were measured, liver sections were observed for pathological evaluation, and Ntcp expression in liver was also examined by Western blotting technique.3. A total of48Wistar male rats were divided into six groups, rats were orally administered with saline and different seaweed extracts, i.e. petroleum ether extract, ethyl acetate extract, ethanol extract, seaweed polysaccharide and decoction, for14days. The dosage given to mice was equal to6g/kg crude herbs. After drugs administration, serum biochemical parameters were measured, liver sections were observed for pathological evaluation, and Ntcp expression in liver was also examined by Western blotting technique.4. A total of24Wistar male rats were divided into three groups:control group, INH-RIF group, seaweed polysaccharide group. Rats in INH-RIF group were orally administered with RIF (100mg·kg-1·d-1) plus1NH (100mg·kg-1·d-1), rats in control group were orally administered with saline, rats in seaweed polysaccharide group were orally administrated with seaweed polysaccharide (720 mg·kg-1·d-1) at3h before RIF (100mg·kg-1·d-1) and1NH(100mg·kg-1·d-1). Drugs were given to rats once a day for successive21days. At21days after drugs administration,each group were fasted for6h. Blood were collected by abdominal aortic puncture, and the serum was obtained for ALT, AST,ALP and TBA analysis. Liver were removed, one part for histological evaluation; one for GSH and MDA level detection, and one for Western blotting analysis to detect the expression of transporter Ntcp.Results1. Compared to the control group, seaweed decoction treatment significantly increased Ntcp expression in the liver of mice by319%(P<0.01); liquorice decoction treatment slightly increased Ntcp expression by14%(P<0.05); while rats treated with liquorice and seaweed mixed decoction at the ratio of1:3,1:1, and3:1, no significant difference was founod in Ntcp expression among these groups (P>0.05), but there is a trend that Ntcp expression was gradually elevated with the increase of seaweed content in the mixed decoctions. Compared to seaweed decoction treated group, liquorice and seaweed decoction treatment significantly reduced Ntcp expression; Ntcp expression was decreased by65.5%,78.2%and77.8%in mice treated with liquorice and seaweed decoction at the ratio of1:3,1:1and1:3, respectively.2. Compared to the control group, seaweed decoction treatment significantly increased Ntcp expression by150%(P<0.01); liquorice decoction treatment slightly increased Ntcp expression by13.9%(P<0.05); while rats treated with liquorice and seaweed mixed decoction at the ratio of1:1, Ntcp expression was decreased by35%(P<0.05). When rats treated with liquorice and seaweed mixed decoction at the ratio of1:3and3:1, there were not significant difference in Ntcp expression (P>0.05).3. Compared to the control group, seaweed decoction and seaweed polysaccharide both markedly increased Ntcp expression with270%(P<0.01) in seaweed decoction group and360%increase (P<0.01) in seaweed polysaccharide group; petroleum ether extract treatment significantly reduced Ntcp expression by27%(P<0.01); while ethyl acetate extract and alcohol extract did not show any effect on Ntcp expression in the liver of rats (P>0.05).4.1NH-RIF group significantly increased biochemical parameter (P<0.01), MDA level (P<0.05) and expression of Ntcp (P<0.01), but decreased GSH level (P<0.05) when compared to that of the control. Seaweed polysaccharide treatment markedly reversed the alterations induced by INH and RIF. Seaweed polysaccharide group significantly decreased biochemical parameter (P<0.01) and MDA level (P<0.05), but increased GSH level and expression of Ntcp (P<0.01) when compared to that of the INH-RIF. Conclusion1. Seaweed decoction can markedly enhance Ntcp expression; and liquorice decoction can slightly elevated Ntcp expression in the liver of mice, while seaweed and liquorice mixed decoction can decrease the expression of Ntcp in liver.2. The impact of liquorice decoction as well as seaweed decoction on Ntcp expression in the liver of rats was as same as that of in mice.3. This present study, for the first time, discovered that two seaweed extracts showed adverse effect on regulating Ntcp expression in the liver of rats, i.e. polysaccharide extract can upregulate Ntcp expression, while petroleum ether extract can reduce Ntcp expression in the liver of rats.4. Seaweed polysaccharide presents hepatoprotective effect in INH and RIF induced liver injury, the underlying mechanism is related to the anti-lipid peroxidation and up-regulation of Ntcp in liver. |
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