Experimental Study On Cartilage Targeting Non Viral Gene Delivery System For Repairing Articular Cartilage Defect In Rabbits

ObjectiveIn vitro cartilage in vivo targeting non viral gene delivery system (LMWC/EGFP); a preliminary study of LMWC/EGFP gene nano complex to theaccumulation mechanism and to observe its promote cartilage repair function in themodel of rabbit articular cartilage defects of the knee cartilage cells induced in target.MethodPreparation of TGF-β1/LMWC DNA nanoparticles group, TGF-β1/PEI DNAnanoparticles group and the two group complex, in the transfection of rabbit articularcartilage cells in vitro, measured by ELISA in0,1H,6h,1D,2D,3D,5D,7d trainingcontent of hTGF-β1in liquid;Preparation of rabbit model of knee articular cartilage defects, preparation ofTGF-β1/naked plasmid complexes as blank control group, three groups ofcomposite (containing green fluorescent protein) were implanted into rabbit cartilagedefect, transfection were killed after2months, the knee joint cartilage tissue forhistological observation, complex aggregation and cartilage the growth of cells influorescence microscopy and immunofluorescence staining of collagen type II.ResultIn vitro transfection group three complexes transfected chondrocytes cultureliquid of TGF-β1concentration decreased, but the experimental group wassignificantly higher than that in the control group,2months of three groups ofcompounds into the articular cartilage defects in rabbits after experimental group,found that compound of TGF-β1/LMWC DNA nanoparticles group the pathologicalsections was significantly higher than that in the control group, and the chondrocyteTGF-β1/LMWC DNA nanoparticles group under fluorescence microscope wassignificantly higher than that of the control group, immunofluorescence, showing thenumber of type II collagen is higher than that of the control group, the differenceswere statistically significant (p<0.05). ConclusionTGF-β1/LMWC DNA nanoparticle complexes in vitro and in vivo caneffectively induce cartilage cells, and because/LMWC DNA nanoparticles compositegroup compared with the TGF-β1/PEI DNA nanoparticles in rabbit articularcartilage defect aggregation increased significantly, so it is considered a charge relatedthis aggregation phenomenon. At the same time, TGF-β1has certain slow releasefunction, which can be used to repair the defect of articular cartilage of the knee.