Ubl-like Protein ISG15-induced Apoptosis In Malignancies

Part1ISG15induces apoptosis of HeLa cells via NF-B pathwayObjectives: To study the effect of ISG15on the apoptosis of HeLa cells andexplore its molecular mechanism.Methods:(1) ISG15reconstructed plasmids and ISG15lentivirus vector wereamplified by PCR;(2) Detection of cells proliferation by cell counts afterover-expression of ISG15;(3) Western blot was performed to examine the expressionof proteins after over-expression of ISG15on HeLa cells that involved in cellapoptosis;(4) would assay was used to determine the effect of ISG15on HeLa cellsmigration.Results:(1) Over-expression of ISG15inhibited HeLa cells proliferation;(2)ISG15induced apoptosis of HeLa cells via NF-B pathway;(3) ISG15resisted HeLacells migration.Conclusion: Over-expression of ISG15could induce apoptosis of HeLa cells viaNF-B signaling pathway. Part2Role of ISG15in the effect of clioquinol-induced blood cancercells apoptosisObjectives: To study the Role of ISG15in the effect of Clioquinol-induced blood cancer cells apoptosis.Methods:(1) Detection of ISG15mRNA and protein expression level after thetreatment of Clioquinol by RT-PCR and Western blot;(2) Effect of Clioquinol onInterferon signaling pathway by RT-PCR;(3) Exploration of the role ofover-expressed ISG15on blood cancer cells proliferation and apoptosis after themediation of lentivirus by MTT assay and Western blot.Results:(1) Clioquinol could significantly induce ISG15expression in bloodcancer cells as well as its de-conjugated enzyme, USP18;(2) Clioquinol may induceISG15through activation of JAK-STAT signaling pathway;(3) Over-expression ofISG15inhibited blood cancer cells proliferation and induced apoptosis of bloodcancer cells.Conclusion: Clioquinol could induce ISG15and over-expression of ISG15induced apoptosis of blood cancer cells.