The Value Of TCT Combined With High-risk HPV In Screening Cervical Cancer And Precancerous Lesions And The Effect Of LncRNA ATB On Proliferation,Invasion And Apoptosis Of Cervical Cancer HeLa Cell Lines

Part one The value of TCT combined with high-risk HPV in screening cervical cancer and precancerous lesionsObjective:To investigate the clinical application of TCTcombined with high-risk human papillomavirus(HPV)-DNA in screening cervical cancer and precancerous lesions in order to provide reference for clinical diagnosis,screening and treatment of cervical lesions(precancerous lesions and cancer).Methods:From January 2018 to may 2019,a total of 378 cases of cervicitis,95 cases of CIN ?,101 cases of CIN ?-? and 45 cases of cervical cancer diagnosed by colposcopic biopsy were selected from the patients in the gynecology and physical examination center of the Affiliated Hospital of North Sichuan Medical College.TCT and high-risk HPV-DNA were detected,and the age and results of various examinations were recorded for statistical analysis.Results:1.the total positive rate of high-risk HPV test in 378 patients was 64.02%.The main age of high-risk HPV infection patients was 40-60 years old,the median age was 48 years old,and the coverage rate was 64.88%.2.In the high-risk HPV negative patients,the detection rate of pathological biopsy was 38.24%,in the high-risk HPV positive patients,the detection rate of pathological biopsy was 78.10%,the detection rate of HPV positive patients was significantly higher than that of HPV negative patients(P<0.01);high-risk HPV There was significant difference in the detection rate of cervical lesions among different types(P<0.01).The highest detection rate of HPV 16 was 91.46%.The pathological results were divided into three groups:chronic inflammation group,CIN ? group and CIN?+group.The positive rates of HPV infection in the three groups were 38.7%,52.6%and 95.2%,respectively.There was a significant difference in the positive rate of HPV infection among the three groups(P<0.01).3.This study showed that the results of TCT showed that ASC-H(atypical squamous cells,excluding high-level squamous intraepithelial lesions)was more able to detect CIN?and above lesions(high-level squamous intraepithelial lesions,cervical cancer)than ASC-US(atypical squamous cells without definite diagnosis significance),the difference was statistically significant(P<0.01).TCT results showed that the diagnostic coincidence rate of HSIL group was higher than that of LSIL group(P<0.01).4.According to the results of pathological biopsy under colposcope,Compared with TCT and HPV alone,the combined detection of HPV and TCT had the highest diagnostic accuracy,sensitivity and specificity,which were 89.42%(338/378),98.34%(237/241),73.72%(101/137),respectively.The difference was statistically significant(P<0.01).Conclusion:High-risk HPV infection is positively correlated with the occurrence of cervical cancer,precancerous lesions and the severity of lesions in women.HPV 16 is the most common subtype of infection in cervical cancer and precancerous lesions.The higher the degree of TCT in the diagnosis of cervical lesions(precancerous lesions and cancers),the higher the degree of histopathological examination in the diagnosis of cervical lesions.TCT combined with high-risk HPV detection can improve the coincidence rate,sensitivity and specificity of detection,which provides better clinical guidance for early screening of cervical lesions and reducing missed diagnosis.Part two The effect of LncRNA ATB on proliferation,invasion and apoptosis of cervical cancer HeLa cell linesObjective:to investigate the effects of long noncoding RNA(long non-coding rna,lncRNA)ATB expression on proliferation,invasion and migration,and apoptosis of cervical Hela cell lines.Methods:the expression of ATB RNA in HeLa cells of cervical cancer was down regulated by small interfering RNA technology,that is,the ATB small interfering RNA(siRNA)and negative control sequence(negative siRNA)were respectively transfected into HeLa cells of cervical cancer,and quantitative real-time PCR(quantitative real-time polymerase chain reaction)was used,QRT PCR)was used to detect the down-regulation efficiency;CCK8 and clone formation experiments were used to detect the effect of down-regulated ATB expression on HeLa cell proliferation;flow cytometry was used to detect the effect of down-regulated ATB expression on HeLa cell apoptosis;Transwell experiment was used to detect the effect of down-regulated ATB expression on HeLa cell invasion and migration.Results:The expression of ATB in Hela cells of human cervical cancer was significantly down-regulated by qRT-PCR(P<0.01);CCK8 and clone formation experiments showed that down-regulated ATB expression significantly inhibited the proliferation of Hela cells(P<0.01);flow cytometry experiments showed that down-regulated ATB expression promoted the apoptosis of Hela cells;Transwell experiments showed that down-regulated ATB expression significantly inhibited the invasion and migration of Hela cells(P<0.01).Conclusion:ATB-siRNA transfection of Hela cells in human cervical cancer can significantly downregulate the expression of ATB in Hela cells.suggesting that long noncoding RNA ATB is an oncogene in cervical cancer,and the abnormally high expression of ATB may be an important molecular mechanism for the development of cervical cancer.