| Ischemia cerebrovascular disease (ICVD) is characterized by highmorbidity, high mortality, high disability and high recurrence rate. Becauseof strong social pressure and fast social rhythm, more and more young andmiddle-aged people have suffered from ischemia cerebrovasculardisease.The rescue and rehabilitation costs increased year by year, whichhave brought serious economic burden to the society, families andindividuals, severely threatening human health and life.Traditional treatment methods consist mainly of early thrombolysis,improve ment of circulation, nourishment of nerves, etc.But the treatmenteffect is not optimistic. Therefore, to explore its proper treatment hasbecome our priority. A large number of clinical studies have shown thatcerebral ischemia in patients with ischemic brain tissue microvasculardensity increased and the survival time of patients were positivelycorrelated to it, the formation of new collateral vessels can improvecerebral ischemia area surrounding tissue perfusion, but the self-reaction ofthe body is still not strong enough to improve the neural function recoveryafter cerebral ischemia. Traditional Chinese medicine has the advantage ofpromoting function recovery after cerebral ischemia microvascular andimproving the mechanism of angiogenesis after cerebral ischemia. Bymaking ischemia-reperfusion rats model,this experiment is to observe theeffect of Kangnaoye on the expression of VEGF, HGF and ES protein andthus to discuss the mechanism of Kangnaoye’s function of anti-ischemic byangiogenesis.186male SD rats were randomly divided into six groups:Kangnaoyetreatment groups (high, middle and low dosage), Nimodipine group, model group and sham operation group. The models of cerebral ischemiareperfusion were established in rats by the methods of middle cerebralartery occlusion (MCAO). Immune histochemical methods are used toobserve brain ischemia area changes of microvascular density (MVD) andVECF, HGF and ES protein expression in brain tissue, and the in situhybridization method to detect the expression of VECFmRNA andHGFmRNA of rats, which were killed at3h,6h,12h,1d,3d,7d of reperfusioninjury respectively2h after cerebral ischemia. It’s to observe theneurological evaluation at2h,24h,48h, the pathological change of rats’brain tissues by the method of HE Staining and the volume change ofcerebral infarction in rats by TTC Staining.Compared to the sham group’s nerve function defect,model group’swas quite obvious after ischemia-reperfusion, its neurons were markedlyswollen, its cerebral infarction volume increased obviously,and theexpression of VEGF, HGF, ES protein and gene rose significantly. Thedifference was significant（P<0.05or P<0.01. After the reperfusion of ratswith cerebral ischemia, it could be noted in each group with administrationof Kangnaoye and nimodipine that the symptom of neurologicalimpairment was eased and the volume of cerebral infarction reduced andneuron swelling mitigated and the expression VEGF, HGF protein and genelevel rose and that of ES decreased. The difference was significant incomparison with the model group(P<0.05or P<0.01). Compared to thenimodipine group, Kangnaoye group with middle and high dosage couldreduce the impairment evaluation of nervous system and the volume ofcerebral infraction and the pathological change of brain in varying degrees.The expression of VEGF, HGF protein and gene was apparently increasedand ES reduced, and the difference was significant (P<0.01). However,there wasn’t significant difference between Kangnaoye group of middledosage and high dosage, which are much better than the low dosage group(P<0.01). In a summary, we can safely draw the conclusion that neurologicalevaluation, infarct volume and pathological changes of Kangnaoye groupscould be improved.Kangnaoye showed the protective effects on cerebralischemia-reperfusion injury in rats, by up-regulating the expression ofVEGF, HGF protein and their gene and down-regulating the expression ofES protein and gene. |
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