Effect Of BFGF On Expression Of VEGF And Ang-1in The Acute Wounds In Rats

Objective: Through the experimental study of basic fibroblast growth factor (bFGF) onvascular endothelial growth factor in wound healing of acute rat(VEGF),angiopoietin1(Ang-1) expression, to further study the effect of basicfibroblast growth factor in promoting the new mechanism of acute wound healing,so as to provide theoretical basis for its clinical treatment.Methods:Through the method of self control study, selection of30male SD rats, in ratson both sides of the spine two cm diameter full-thickness wound model wasestablished, the left side as experimental group, the daily physiological salinedebridement, bFGF wet dressing change processing; on the right side as controlgroup, the daily physiological saline debridement, saline wet compress dressingtreatment.And then at1st,3rd,7th,10th,14th randomly6rats were killed, and takeon both sides of the spine of wound tissue, routine HE staining was used to observethe pathological morphology and detection in different time point expression ofwound tissue in rats of two groups of VEGF, Ang-1by immunohistochemicalmethod.Results: The wound of rats14days after injury healing wounds, the control group ratswere still cover, are not entirely covered with epithelium. VEGF and Ang-1immunohistochemical changes of expression in the experimental group and thecontrol group during the process of wound healing is consistent, all is first days after injury began to rise, about7th days after injury and reached the peak, thendecreased gradually. The experimental group VEGF and Ang-1in third after injury7th,10th compared with the control group had some differences, including aseventh day after injury, there were significant differences, and in first days afterinjury and fourteenth days after injury was not significantly different.Conclusions:1.In the process of back wound healing in rats, the expression of VEGFandAng-1trends and wound healing angiogenesis process in the time consistent,indicating that VEGF and Ang-1are closely correlated to the wound healing, mayparticipate in the coordination plays an important role in regulating angiogenesisduring wound.2. The experimental group of acute wounds in early VEGF, Ang-1expression wassignificantly increased, while the control group, the expression relative delay, andthe expression peak of the experimental group is low, and VEGF is pro angiogenicpromoter, so that VEGF may initiate angiogenesis, and further induce Ang-1expression, and promote the formation of new blood vessels from.3.The experimental group was significantly faster than the control group, suggestingthat bFGF can promote wound healing, and its mechanism may be related withbFGF can enhance the expression of VEGF, VEGF can increase synergisticallyinduced Ang-1expression,so as to effectively promote wound angiogenesis, andfurther stimulate the wound granulation formation, thus accelerating the acutewound healing.