| With the acceleration of social development, and continuously improve the quality of life, therequirements that people ask for treament of various diseases are increasingly high, Especially fortissue or organ repair,the common methods of traditional clinical medicine are autograft andallograft. However, due to lack of donor and probably induce different kinds of complications, itcan hardly satisfy the needs of patients. Tissue engineering in particular additive manufacturingtechnologies emerge, providing new ideas for solving this problem.This paper was divided into two parts, respectively three-dimensional controlledcell-assembling scaffold of artificial larynx cartilage and animal experiment research of PLGAscaffolds. The first four chapters tell the research of three-dimensional controlled cell-assemblingtechnology and the preparation of laryngeal cartilage, the chapter5describes the experimentalabout using PLGA scaffold made by low-temperature forming technology to repair spinal cordinjury.Three-dimensional controlled cell-assembling technology develop by fusing life science,materials science, computer science and other fields. It is an important branch of additivemanufacturing technology that is rapid prototyping technology. Compared with other additivemanufacturing, it can print activated scaffolds directly that is cells scaffolds. The environment ofscaffolds preparation doesn’t cause any damage to cells, it is a kind of ideal additive manufacturingtechnology. Recently, as the aggravation of air pollution, the morbidity of laryngeal cartilagedisease increased gradually, the traditional way that using laryngeal transplantation far behind thedemand, so using cells controlled directly assembly technique to produce laryngeal cartilagescaffolds directly provide new way to laryngeal cartilage injury repair. First, this paper analyze eachcurrently optional cartilage tissue engineering materials first to compare their respective advantages,then it combined with cells controlled assembly technique and eventually choose gelatin andsodium alginate composite material that have good histocompatibility, biocompatibility, mechanicalperformance and appropriate degradation rate. Secondly, this paper has analyzed the principle andcomposition of cells controlled assembling machine and set each parameters of system according tothe experiment experience. Then it has studied the material matching concentration ratio andproduce high porosity, moisture content, appropriate degradation rate and inflation rate ofgelatin-sodium alginate scaffolds, it fully conform to the requirement of laryngeal cartilagescaffolds. Finally improved type II collagenase digestion method is used to extract high purity andconcentration of chondrocytes, and this paper has studied the characteristics of the cartilage cell batches, it has laid the foundation of subsequent laryngeal cartilage blending culture and animalexperiments.In this paper, the second part of the research content is using clod forming technology toproduce PLGA scaffolds and Schwann cells that have high porosity, good hydrophilicity andappropriate degradation. The PLGA scaffolds and Schwann cells were cultured in vitro after aperiod of time, after that, we begun the animal experiments of scaffolds repair spinal cord injury.First, the rats were divided into four groups and then three group were got the treatment of spinalcord injury, group A were implanted scaffolds and Schwann cells to repair spinal cord injury, groupB were implanted PLGA scaffolds to repair only, group C didn’t get the repair of spinal cord injury,and group D didn’t get the treatment of spinal cord injury and grow regularly. After one month ofregular feeding, we would observing the condition of rats’ recovery and make a BBB score everyweek. Second, we would make tissue section of spinal cord injury from each group and biopsy ofCaspase-3, GFAP, fast blue, and silver dye dyeing respectively to each tissue section, then weanalyzed the condition of rats’ spinal cord injury recovery. According to the last results, therecovery of group A is much better than other groups. Therefore, PLGA with Schwann scaffoldshave good function to promote the recovery of spinal cord injury. |
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