| Objective:To observe in vitro ginsenoside Rg3, sorafenib, oxaliplatin, fluorouracil mono therapy and different joint approach on human hepatoma SK-HEP-1 cell proliferation, apoptosis and of Wnt/β-catenin signaling pathway related protein expression. Chemotherapy drugs, molecular targeted drugs, the combination of traditional Chinese medicine to provide experimental evidence.Methods:First, to observe the effects of drugs on hepatic carcinoma SK-HEP-1 cell growth. Choose different concentrations of ginsenosides Rg3, sorafenib, oxaliplatin, fluorouracil alone act on human hepatoma SK-HEP-1 cells, select the appropriate concentration of the drug at different times according to its inhibition of proliferation combination with each other, Observation of the two drugs, three drugs, different joint approach four drugs on the role of human hepatoma SK-HEP-1 cells.the main experimental methods are:(1) MTT was observed in each group with different concentrations of drugs on the SK-HEP-1 inhibition of cell proliferation; (2) flow cytometry, in situ end labeling (TUNEL) detected in each group in SK-HEP-1 cells. (3) Western blot method (Western blotting) testing groups Wnt/β-catenin signaling pathway key factor Wnt-1, β-catenin and apoptosis-related protein expression of Bcl-2.Results:(1) ginsenoside Rg3, sorafenib, oxaliplatin, fluorouracil monotherapy and combined in different ways can effectively inhibit the proliferation of SK-HEP-1 cells, and this inhibition in time and dose; which of the two drugs and three drugs combination is superior to monotherapy, four drugs combination is superior to two drugs and three drugs combination (P <0.05), mainly among drug for additive or synergistic effects; (2) Flow cytometry situ end labeling (TUNEL) detected in each group SK-HEP-1 cell apoptosis results:each drug group were able to effectively induce SK-HEP-1 cell apoptosis, induction of apoptosis of the two drugs and three drugs combination group superior to monotherapy group, four drugs combination group than the two drugs and three drugs combination groups (P<0.01); (3) Western blotting was used to detect drug effects on SK-HEP-1 cells Wnt-1, β-catenin after 48h, Bcl-2 protein expression results showed that:ginsenoside Rg3, sorafenib, oxaliplatin, fluorouracil monotherapy and combined in different ways can reduce the SK-HEP-1 cells Wnt-1 protein levels. Compared with the negative control group, the experimental group Wnt-1 protein levels were lower (P<0.01 or P<0.05); compared with the monotherapy group, the two drugs and three drugs combination group was reduced Wnt-1 protein levels (P<0.01 or P<0.05); and monotherapy groups, two drugs and three drugs combination group compared to the four drugs combination group Wnt-1 protein levels were lower (P<0.01 or P<0.05). Ginsenosides Rg3, sorafenib, oxaliplatin+fluorouracil monotherapy and combined in different ways can reduce the SK-HEP-1 Wnt-1 protein levels in cells. Compared with the negative control group, the experimental group, p-catenin protein levels were reduced; compared with the monotherapy group, the two drugs and three-drug combination group β-catenin protein levels were lower (P<0.01 or P<0.05); and monotherapy groups, two and three drugs combination drug group compared to four drugs combination group P-catenin protein levels were lower (P<0.01 or P<0.05). Ginsenosides Rg3, sorafenib, oxaliplatin, fluorouracil monotherapy and combined in different ways can reduce the SK-HEP-1 Bcl-2 protein levels in cells. Compared with the negative control group, the experimental group Bcl-2 protein level were lower (P<0.01 or P<0.05); compared with the monotherapy group, the two drugs and three drugs combination group Bcl-2 protein level were lower (P<0.01 or P<0.05); and monotherapy groups, two drugs and three drugs combination group compared to the four drugs combination group Bcl-2 protein level were lower (P<0.01 or P<0.05).Conclusion:Ginsenoside Rg3, sorafenib, oxaliplatin, fluorouracil monotherapy and different joint approach could inhibit proliferation and induce apoptosis of hepatoma SK-HEP-1 cells, the two drugs is better than a single drug, three the drug is better than the two drugs, and drug and strong in four three-drug, combined with the performance of their drug between a good additive or synergistic effects. The specific mechanism may pass down Wnt/β-catenin signaling pathway key factor Wnt-1, the expression of apoptosis-related protein expression of Bcl-2 of P-catenin in a certain relationship, worthy of further study. |
PDF Full Text Request