| Background and Objectives:Liver metastasis in colorectal cancer patients is common, it greatly influences the postoperative survival of patients and leads to bad prognosis. However, early diagnosis of liver metastasis in patients are lack of sensitive tumor markers. Although the treatment of surgical resection can prolong survival time of patients, but low resection rate, difficulty to complete resection and lack of effective targeted therapy drugs leads to bad effects. Liver metastasis of colorectal cancer pathogenesis is very complex, involving multiple genes, chemokines, cytokines and multiple cellular pathways, etc. By far the research on its mechanism has not been fully elucidated, it is greatly helpful to reveal the influence factors of colorectal cancer liver metastasis. Studies confirm that the expression of OPN mRNA and its corresponding protein in colorectal cancer agglomerates in the organization and its expression in colorectal mucosa tissues is low. Moreover, it is participation in colorectal cancer from benign to malignant. CD44 is one of the receptor of OPN, and it is expressed by the CC in the original site(+)and has higher expression rate. What’s more, the transfer of mesangial lymphoid tissue expression rate also increases, and the expression in colorectal mucosa is on the contrary, the research results show that CD44 involved in cell migration, after activating various signaling pathways, and increases the risk of tumor cell proliferation and distant metastasis.Based on the results of research above, we speculated that OPN have a certain correlation with the expression of CD44. It is associated with the malignant degree and the staging of tumor, which can guide patient’s prognosis and targeted therapy. The results of the study are expected to further clarify OPN and the relationship between CD44 and clinical pathology and their correlation and the prognostic significance, which provides new targets for clinical treatment of colorectal cancer liver metastases.MethodsThe expression of OPN and CD44 in 76 cases of colorectal cancer tissues, 30 para carcinoma than 10 cm normal mucosa, 16 patients with liver metastasis tissues was examined by immunohistochemical SP staining method, using statistical software SPSS19.0 for processing,postoperative comparison between groups by χ2 test, P < 0.05 were statistically significant differences. Patients with 5-year survival rate with Kplan-Meier curve were analyzed, and the comparison to express its influence on prognosis.Results:The expression rates of OPN in normal mucosa, colorectal cancer tissues and liver metastatic cancer tissues were 6.7%(2/30),69.7%(53/76),75.0%(12/16) respectively. The expression rates of CD44 in normal mucosa, colorectal cancer tissues and liver metastatic cancer tissues were 16.7%(5/30),52.6%(40/76),68.8%(11/16) respectively. The same as OPN, the expression rates ofCD44 in colorectal cancer and liver metastatic cancer was significantly higher than those in the normal mucosa(P<0.001).The expression of OPN and CD44 was related to infiltration depth, lymph node metastasis, and distant metastasis. There was no significant difference of CD44 expression in the differentiation degrees. High expressions of OPN and CD44 in primary tumors, especially in liver metastases of colorectal cancer have obvious correlation. Five-year survival rate of patients, who express OPN and CD44 positive in colorectal cancer was lower than those of OPN and CD44 express negative.There is significant difference(P < 0.05).Conclusion:In colorectal cancer tissues, the results of OPN and CD44 expression is high; in its liver metastasis tissues, the expression rate is also high, and the expression rate in these two organizations is significantly higher than the corresponding expression in normal tissue. Therefore, both in high expression rate of primary tumors and metastatic carcinoma tissue s are closely related to local invasion and distant metastasis of malignancy. Both of them play an important role in the process of the development of malignant tumor. In addition, both of the high expression rate has certain correlation. The statistical analysis shows that there is interaction between the two proteins, increase in tumor development and metastasis. The patients with positive expression of OPN and CD44 have poorer prognosis than patients with those of both express negative. It shows that the expression and prognosis is closely related to the patients. The two proteins can screen patients with high risk, so that doctors can take appropriate treatment for individual as soon as possible. |
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