The Inhibitory Effect Of Docetaxel On Human Hepatocellular Carcinoma Subcutaneous Xenografts In Nude Mice

Objective To disguss the effect of docetaxel on the growth of human hepatocellular carcinoma cell line subcutaneous xenografts in nude mice and the possible mechanism..Methods SMMC-7721 hepatocellular carcinoma subcutaneous xenografts were established in nude mice, after the success of modeling,20 nude mice were randomly divided into two groups,docetaxel group and control group with 10 rats each.The experimental group were injected with 50ug.3ml-1 docetaxel once every 3 days for consecutive 7 times.The control group were injected with 50ug.3ml-1 saline as mentioned above.The changes of body weight, tumor volume and general condition were observed continuously in two groups during the whole experiment. After 3 weeks,dealing with the nude mice,the tumor weight and volume were measured.The apoptosis of tumor cells and hTERT, Ki67, VEGF and P53 positive expression rate of each group were determined respectively through Tunel and immunohistochemical method.ReSUltS After docetaxel treatment, the experimental group of the tumor growth slowed down, naturely the final tumor volume and weight were also lower than that of the control group, and the difference were statistically significant (P<0.05).Body weight in experimental group was no significant difference before or after the treament(P<0.05).The index of apoptosis of tumor cells in the docetaxel group was significantly higher than that of the control group(P<0.05). The experimental group of hTERT, Ki67,VEGF and P53 positive expression rate were significantly lower than that of the control group(P<0.05).Conlusion Docetaxel can inhibit the growth of hepatocellular carcinoma subcutaneous xenografts in nude mice. The mechanism of inducing the apoptosis of tumor cells and inhibiting the proliferation of hepatoma cells are associated with the reduction of Htert, Ki-67and P53 protein expression. In addition blocking the migration of vascular endothelial cells is raleted with reducing the expression of VEGF protein. Docetaxel in the concentration of treatment range has no toxicity on mice.