The Research Of Physical Properties Of Nanobubble And Biological Mechanisms Of Anesthesia

Nanobubble has been studied more than 20 years. It has been found that nanobubble has the important application in physic, chemistry, biology and the cross discipline of the two or three. At present, on both theoretical research and experimental operations there was not a unified conclusion of long-term stability at solid/liquid interface. Especially, the study of theoretical aspects was very scarce. It was found that the wetting properties of the substrate were one of the important factors of the nanobubble stability adsorption at the solid/liquid interface. To fully understand nanobubble stability characteristics at different substrate surface, we provided two fixing the nanobubble ways based on the molecules dynamic simulation (MD):(1) from the special arrangement of the charge and charge dipoles to change the wetting properties of the graphene surface, the special area of graphene surface that the center area was hydrophobic surround by the hydrophilic area. The nitrogen nanobubble was easily fixed at the hydrophobic area surrounded by hydrophilic area; (2) In the center area of the graphene surface modified with an arrangement of the charge and charge dipoles, the wetting properties of modified area was similar with the area without modified. Because of the effect of modified area with hydrophilic edge (water wall) and pre-placed gas, the nitrogen nanobubble was also easily fixed at this modified area. Those results can be helpful to explain the stability and application of the nanobubbles in microfluids.Xenon was an anesthetic gas without any negative effects on the human body. Its effect on clinical above was very gratifying. The xenon was an inert gas, which could not have any chemical action in human body. Thereby, the anesthetic mechanism of xenon has not been very reasonable explanation now. A new thinking that xenon nanobubbles were proposed and the nanobubble could be adsorbed on the one site of human Protein molecules. The area would be out of function, which can produce the effect of anesthesia. We used fluorescence absorption spectrum and fluorescence imaging techniques of synchrotron radiation X-ray to study the adsorption of xenon on pepsin surface in the BL15U1 and BL14W1 of SSRF. Preliminary results showed that xenon molecules adsorption of the pepsin were higher than the aqueous solution without protease. In other words, micro/nanobubbles of xenon may be adsorbed on pepsin surface. The experiment studies will help to reveal the microscopic mechanism of xenon anesthesia.