Analytic Study Of Non-UV Absorption Nitrogen-Containing Drugs By High Performance Liquid Chromatography Based On Charge Transfer Reaction

Charge transfer complexe (CTC) is a class of complex formed between electron donor and electron acceptor through charge transfer reaction. The CTC has been widely applied in various field including chemistry, biomedicine, pharmacy, etc. In recent years, its application in parmacuetical analysis catches extensive attention.In the precent work, we screen and optimize contributing factors such as electron acceptor species, medium of charge transfer reaction, pH and volume of buffers, reaction temperature and time, and volume of electron acceptor with spectrophotometric method, establishing the quantification of non-UV absorption nitrogen-containing drugs, including L-cysteine, L-leucine, L-threonine, amikacin, etimicin and netilmicin by spectrophotometry and high performance liquid chromatography (HPLC) based on charge transfer reaction. Furthermore, the composition, molar absorptivity and stability were measured and the mechanism of CTC formation was also preliminarily studied.This work optimize the reaction conditions between non-UV absorption nitrogen-containing drugs and electron acceptor quinones with spectrophotometric method, and introduce the charge transfer theory in pre-column derivatization of HPLC, establishing a new method for content measurement of non-UV absorption nitrogen-containing drugs.