Study On The Chemistry Constituents And Bioactivity Of Cercis Chinensis Bunge And Angelica Dahurice

This dissertation is composed of three chapters.In the first chapter, antioxidant activityand α-glucosidase inhibitiory activity of 70% ethanol extracts from Cercis chinensis Bunge flowerswere investigated with Bioassay-guided fractionation. And the antioxidant, α-glucosidase inhibitory and anti-bacterias activity of different extracts, fraction and compounds from C. chinensiswere assayed.The second chapter studied the antioxidant activityand α-glucosidase inhibitiory activity of 70% ethanol extracts from Angelica dahurice Benth.were investigated with Bioassay-guided fractionation. And the antioxidant, α-glucosidase inhibitory and tyrosinase activity of different extracts, fraction and compounds from A. dahuricewere investigated. The third chapter, the progress of chemical constituents and pharmaceutical of the genuswere summarized.Chapter 1. Antioxidant and α-glucosidase inhibitiory activity of Cercis chinensis flowersIn this study, active compounds of 70% ethanol extracts from C. chinensis flowerswere investigated with Bioassay-guided fractionation.The chemical constituents from theC. chinensis were studied by column chromatography. Eight compounds were identified from active fractions asethyl gallate(1), stearic acid(2), docosanoic acid(3), 5α-stigmast-9(11)-en-3β-ol(4), kaempferol-3-O-α-rhamnopyranoside(5), vanillic acid(6), fisetin(7), and β-sitosterol(8). And compound 2~6 and 8 were isolated for the first from the genus.Different extracts, fraction and compounds of 70% ethanol extracts from C. chinensis flowers were assayed for α-glucosidaseinhibitory effect by the 96-microplate-based method and compared with acarbose as positive control. Ethyl acetate fraction(CLEa) and n-butanol fraction(CLBu) exhibited α-glucosidase inhibitiory activity in vitro, and the corresponding active fractions, EaFr.4, EaFr.6, BuFr.1, BuFr.2, and vanillic acid, exhibited higher α-glucosidase inhibitiory activity. The antioxidant activities of different extracts, fraction and compounds of C. chinensis flowers were assayed by the DPPH, ABTS and FRAP methods. Ethyl acetate fraction(CLEa) and n-butanol fraction(CLBu) exhibited antioxidant activity in vitro, and the corresponding active fractions, EaFr.5, BuFr.1, Bu Fr.2, and ethyl gallate exhibited higher antioxidant activity than that of other fractions and compounds.Chapter 2. Antioxidant and α-glucosidase inhibitiory activity of Angelica dahuriceIn this study, active compounds of 70% ethanol extracts from A. dahurice were investigated with Bioassay-guided fractionation.The chemical constituents from the C. chinensis were studied by column chromatography. Eight compounds were identified from active fractions aspalmitic acid(1), 2′-deoxymeranzin hydrate(2), pabulenol(3), isogosferol(4), heraclenol(5), and stearic acid(6).Different fractions and compounds of 70% ethanol extracts from A. dahuricewere assayed for α-glucosidase inhibitiory activityby the 96-microplate-based method and compared with acarbose as positive control. The corresponding active fractions, Fr.5, Fr.6, Fr.7, exhibited α-glucosidase inhibitiory activity in vitro, and compound pabulenol(3) and isogosferol(4) exhibited higherα-glucosidase inhibitiory activity. The activity of tyrosinase was detected of different fractions and compounds of 70% ethanol extracts from A. dahuricewith Bioassay-guided fractionation. And the fractions with a good tyrosinase activated activityin vitro. Palmitic acid(1), pabulenol(3) and isogosferol(4) showed stronger inhibitory effect against tyrosinase, and heraclenol(5) has a activated activity of tyrosinase.Chapter 3. Advances of chemical constituents and pharmacology of Cercis Linn.Progress of chemical constituents and pharmacological activities of Cercis Linn. were summarized.