Extraction And Separation Of Effective Components And Pesticide Residues In Traditional Chinese Medicine By Micellar System

Traditional Chinese medicine has been employed widely in preventing and curing mumerous disease for thousands of years. As the active components in the traditional Chinese medicine formed the material base of its pharmacological effects, establishing a rapid and effective method to extract the components is of significant importance. Additionally, the quality of traditional Chinese medicine has come to greater concern along with the increase of its demand on the market. In order to ensure the quality and production of the medicine plants, many kinds of pesticides were necessarily used during their cultivation. Although increased the production of the plants remarkably, pesticides necessarily caused residue problems. Therefore, establishing a rapid and effective method to detect and analyze pesticides residue in traditional Chinese medicine to ensure safe application is of great urgent.A micellar system formed by the non-ionic surfactant Triton X-114 in water solution was used in this paper to extract and separate the active components or pesticide residue in traditional Chinese medicine. The main contents were as follows:1.The extraction and separation of bergenin from Ardisia japonica was performed using the micellar system formed by the non-ionic surfactant Triton X-114, and the enrichment of bergenin was achieved by cloud point extraction. Factors affecting extraction rate of the bergenin during the experiment were inspected, thus the optimum conditions for the extraction of bergenin from Ardisia japonica were obtained. And the method was successfully applied to the extraction of berenin from Ardisia japonica grew at different areas.2.The extraction of the non-water-soluble active component camptothecin from Camptotheca acuminate was performed using the micellar system formed by the non-ionic surfactant Triton X-114 in water solution, and the enrichment of camptothecin was achieved by a water bath process. Affecting factors during the experiment were inspected, and the kinetics during enrichment process was fully researched. This method was then applied to the extraction and separation of camptothecin from different parts of Camptotheca acuminata.3.The extraction and enrichment of four kinds of pyrethroids residue from traditional Chinese medicine was achieved using the micellar system formed by the non-ionic surfactant Triton X-114 in water solution, and the purification was completed by adding activated carbon during enrichment process. Affecting factors during the experiment were inspected, and this method was applied to analysis of pyrethriods residue in different traditional Chinese medicine.Through analysis by high performance liquid chromatography, under the optimus conditions, the obtained extraction yield of bergenin from Ardisia japonica was 0.53%, with a precision (RSD) of 4.1%(n=5) and detection limit of 0.65μg·mL; the obtained extraction yield of camptothecin from Camptotheca acuminata was 0.1157%, with a precision (RSD) of 5.4%(n=5) and detection limit of 0.7μg·mL-1. The recoveries of four kinds of pyrethroids are 72.4%-94.3%, with precision (RSD) of 3.2%-6.7% and detection limit of 0.0083-0.0108μg·g-1, which indicates satisfacting sensitivity and repeatability.The method of separate and extract active components and pesticide residue in traditional Chinese medicine by the use of micellar system which proposed in this paper was indicated by these mentioned results to have the advantages of simple operation, controllable condition, no organic solvent usage, therefore of low cost, non-toxic and environmental-friendly.