A Clinical Research On Chronic Non-atrophic Gastritis(spleen Stomach Damp Heat Type)treated With Huashi-qingre-anzhong Decoction Combined With Domperidone And Omeprazole And The Effect Of AQP3、AQP4

Objective: This paper based on previous studies,To observe the effect of the wet heat Ann prescription in Treating Chronic Non-Atrophic Gastritis( CNAG) due to spleen stomach damp heat type;To evaluate the influence on the gene and protein expression of AQP3、AQP4 and on the pathological effect of gastric mucosa.Methods: The 50 patients with CNAG due to spleen stomach damp heat type, according to the visiting sequence were randomly divided into treatment group and control group. Of which 25 cases of treatment group, 25 cases in the control group.The treatment group was given the Huashiqingre-Anzhong Decoction,omeprazole and domperidone Tablets and while the control group the latter was given Omeprazole and Domperidone,Each patient was given continuous medication for 4 weeks.Statistical TCM syndrome integral before and after the treatment.Statistical integral of gastric mucosa after the treatment.With HE staining method detecting the pathology effect of gastric mucosa,Immunohistochemical method was applied to detect the expression of AQP3,AQP4 protein and RT-PCR method to gene.Results:(1)The two groups after treatment of TCM syndrome integral were lower than those before treatment,the difference was significant(P<0.05),suggesting that two groups of drugs can improve the syndromes of traditional Chinese medicine;the two groups before and after treatment of TCM syndrome integral difference comparison,scores of treatment group difference is greater than the control group(P<0.05),the treatment group to improve the syndromes of traditional Chinese medicine is better than that of the control group;two groups of therapeutic efficacy of TCM syndromes distribution and the total efficiency of the comparison,the difference was significant(P <0.05),the treatment group of TCM Syndromes of efficiency is 96%,control group 84%;the two groups after treatment the symptoms integral and compared before treatment,there were significant differences,the two groups before and after treatment in P<0.05;epigastric fullness distension or pain,nausea and vomiting,stomach symptoms integral difference comparison,no significant difference, P>0.05,in comparison with thick and greasy tongue coating,dry bad breath and stool sticky symptom integral,p<0.05.The treatment group in improving symptoms of patients with damp heat better than the control group.(2)No significant difference was found between the two groups patients with gastroscopy,P>0.05.(3)Comparison of gastric mucosa pathological morphology in the two groups after treatment,the treatment group improved better than control group in gastric mucosal inflammation,the difference was significant(P<0.05).(4) After treatment,the average optical density value and gene expression of AQP3、AQP4 of the two groups decreased significantly(P<0.05), the treatment group was significantly decreased compared with the control group, with statistically significant difference(P<0.05).(5) Patients in both groups could tolerate and no case of obvious adverse reactions.Conclusion:(1) The clinical symptoms,especially for thick and greasy tongue coating,dry bad breath and stool viscous symptoms improved significantly better than the control group of Western medicine.(2) Huashiqingre-Anzhong Decoction improve the Clinical Symptoms and reduce the expression of protein and gene of AQP3、AQP4 than single Omeprazole and Domperidone.(3)Huashiqingre-Anzhong Decoction may through improving the protein and m RNA expression of AQP3、AQP4 and improving the inflammatory reaction of gastric mucosa to promote water metabolism and repair gastric mucosal,so as to alleviate or cure the clinical symptoms of patients.(4)The long-term efficacy and the specific mechanism requires a large sample of experimental and clinical study on further.