Research On Effects Of EGCG From Tea On Regulating Lipid Metabolism And Alleviating Liver Injury In Rats With Non-alcoholic Fatty Liver Disease

Non-alcoholic fatty liver disease (NAFLD) was a pathological metabolic syndrome performed as the fat accumulation and/or fatty degeneration in the liver, which was di-rectly relevant to lipid metabolic disorder. Epigallocatechin gallate (EGCG) was the largest fraction of the catechin in the tea, and also reported as the main bio-active ingredient of the tea. Recently, many research showed that many bio-functions of tea were related with EGCG, such as anti-oxidation, anticancer, antiradiation, hypolipidemia and weigth reduction, but the effective mechanism was unclear. In this study, EGCG was taken as the research material, through experimental rat model to investigate the effects of EGCG from tea on regulating lipid metabolism and alleviating liver injury in rats with NAFLD.1. EGCG on regulating lipid metabolism and alleviating liver injury in rats with NAFLDFirstly, NAFLD model rats were established by feeding a high fat diet for28days, then the rats of EGCG protective groups were given daily oral administration of EGCG with three doses of10,50and100mg/kg-BW for another35days, respectively. After35days, evaluating the effect of EGCG through measuring the body weight, blood fat, anti-oxidative ability of liver and pathological changes of liver. The results showed that, animal model was sucsessful after feeding a high fat diet for28days. And compared with the model group, EGCG could decrease serum levels of triglyceride(TG), total cholesterol(TC) and low density lipoprotein-cholesterol(LDL-C); increased serum level of high density lipoprotein-cholesterol(HDL-C). Activities of alanine aminotransfer-ase(ALT) and aspartate aminotransferase(AST) were reduced in the serum, and also ameliorated liver oxidative stress. Histological analysis showed that EGCG could reduce the formation of lipid droplets, inflammation and hydropsia were improved in liver.2. Research of gene expression of EGCG regulating NAFLD rats lipid metab-olism in liver Real-time fluorescent quantitive PCR was used to detect the expression of ACAT1、DGAT2、LCA、UCP2. MCD、CPT-1of rats in the normal control, model control and high dose of EGCG group.The results showed that,compared with model control group, EGCG could down-regulate expression of LCAT, ACAT1, DGAT1,UCP2. And it could also up-regulate expression of MCD and CPT-1.These gene expression analysis showed that EGCG could regulate the expression of genes involved in lipid metabolism.3.Proteomics research on EGCG ameliorating NAFLD of ratsThe liver tissue of normal control group, model control and High dose of EGCG group were taken as sample respectively. Through establishiing a2-DE method to sep-arate the differential expressed proteins, and used biologial material spectrum to iden-tify those proteins. The results showed that, agood2-DE method with reproducibility and high resolution was established.34protein spots were found with stable expression in all three groups and abundance changes more than1.5times,13spots were up-regulated and21spots were down-regulated.13spots were picked out to use bio-logical mass spectum to identify, and10spots were sucessfully identified. These10proein were related with lipid synthsis, transport and oxidation.Taken together, these experiment results indicated that EGCG from tea effectively alleviate lipid metabolic disorder and lipid-induced liver injury.