The Anti-tumor Activity Of The Combination Of Matrine And Oxymatrine To Human Hepatoma HepG2Cells And The Impact To Energy Metabolism

In the1990s, people begin to pay close attention to matrine and bitter and alkali oxide, its in vitro and in vivo antitumor effect has a large number of documents. However, like many Chinese medicine effective component, matrine and oxidation and alkali dosage is still large, on normal somatic cell toxic side effects, large dose not conducive to the application. In present anticancer prescription matrine and bitter and alkali oxide exist at the same time, whether the two have synergy. Therefore, we adopt the method of combination, to a certain extent conforms to the general law of compatibility of Chinese medicine, reduce the matrine and oxymatriner, in order to realize the attenuated synergistic effect. Bitter and alkali and matrine is discussed in this paper, the oxidation of human liver cancer cell HepG2, human umbilical vein endothelial cells ECV304energy metabolism, to study traditional Chinese medicine (TCM) on tumor cell energy metabolism effect and inhibition of tumor angiogenesis play a prompt action.This experiment by HepG2in vitro human liver cells and human umbilical vein endothelial cells ECV304, determined by MTT method is used to test drugs on liver cancer cell proliferation inhibition of HepG2, the results show that the matrine and oxymatrine have the proliferation inhibition of the two kinds of cells. Group legal determination two drugs concentration ratio, under the joint application of proliferation inhibition was stronger than the use of matrine or oxymatrine alone. Cell counting method the change of the drugs on the cell growth curve drawing, drug intervention group has inhibitory effect on the growth of the two kinds of cells, and joint suppression is stronger than bitter and alkali oxide group is better than matrine groups. Using HE staining to observe the influence of human liver cancer cell HepG2, human umbilical vein endothelial cells ECV304morphology change, the degree of morphological changes is joint group, observed the nuclei of drug plasma ratio decrease, highly pyknotic nuclei, dyeing degree deepening, chromatin gathered themselves together, and fracture. With transmission electron microscopy shows that after the treatment, further part cell nuclear membrane bulging outward, cavitation of cytoplasm with size, the decrease in the number of microvilli, mitochondria, rough endoplasmic reticulum and other organelles, cells apoptosis.In the study of energy metabolism, cellular protein concentration was measured using the BCA method, the standard curve, the standard equation derived protein concentration. By detec tion kit hexokinase (HK) vitality, and the results showed that matrine, oxymatrine and their co mbination significantly increased liver cells hexokinase vigor, had no significant effect on vase ular endothelial cells.Use of pyruvate kinase (PK) kit detected, matrine and joint inner hepatom a cell pyruvate kinase activity decreased for ECV304cells had no significant effect. Detection kit using succinate dehydrogenase (SDH) activity found in the drug had no effect on liver cane er cells SDH activity, oxymatrine, endothelial cells in the control group SDH activity decreased significantly. Kit to detect lactic acid (LD) content, the results showed that all groups on endot helial cell culture medium had no significant effect of lactic acid accumulation, liver cell cultur e medium significant accumulation of lactic acid. In the determination of the total cell ATP con tent experiments, ATP content was found in each group decreased endothelial cells, liver cells, only the combination group showed a significant downward trend.