Experimental Study On Bone Defect Repair Materials Alpha-CSH/BCP About In Vitro Degradation And Cell Compatibility

[Objective]:By simulating the in vivo environment of new compound calcium sulfate artificial bone alpha hemihydrate calcium sulfate/biphasic calcium phosphate ceramic (alpha-CSH/BCP) in vitro mechanical properties, degradability and cytocompatibility, preparing for the further animal experiments.[Method]:Alpha-CSH and BCP after binding for the development of new alpha-CSH/BCP artificial bone, The alpha -CSH, BCP and alpha -CSH/BCP were defined as group A:alpha -CSH/BCP group materials; group B:Alpha-CSH Group materials; group C:BCP group materials. To simulate in vivo environment in vitro respectively by mechanical property test, in vitro degradation, leaching solution pH determination of cell adhesion and growth, comparative study, evaluation of mechanical properties, degradation properties and cell compatibility of the composite.[Result]:The results of analysis were entered into each material:Three groups of material mechanical test results show that:alpha-CSH/BCP group was significantly higher than that of compressive capacity of alpha-CSH group and BCP group; Three groups of Materials Determination results of weightlessness rate display:second weeks, alpha-CSH/BCP group and BCP group than alpha-CSH is slightly faster (P<0.05), the BCP group is relatively slow; three groups of materials with the extension of time, the weight loss rate of alpha-CSH group increased significantly, while the alpha-CSH/BCP group and the BCP group decreased weight loss, weight loss is still slow; Three groups of material leaching results determination of extract pH shows:with the extension of time, the pH value change in the three groups of materials are not. Alpha-CSH acid, in the decomposition process because of increased concentration of the acid strength, while BCP is weakly alkaline, in the decomposition process, the alkaline increased gradually, while the alpha-CSH/BCP group because of weak alkaline alpha-CSH acid and BCP in the decomposition process of continuous neutralization, somewhere between its pH value. Results the three groups showed that growth of osteoblast; and around the alpha-CSH materials see a small amount of the growth of osteoblasts. Also in the scanning electron microscope could be seen under the alpha-CSH/BCP and BCP materials on the adhesion of many osteoblasts, and alpha-CSH only a small amount of osteoblast cell adhesion.[Conclusion]:In the simulated in vivo environment experiment, mechanical properties,degradation properties and cell compatibility of new alpha-CSH/BCP canobviously better than alpha -CSH and BCP, with a fine application foreground.