The Expression Of AFP And DLK1 In Experimental Hepatocarcinogenesis In Rats And Human Serum

[Objective]To study different expressions of AFP and DLK1 in hepatocellular carcinoma of model rats and human serum.[Method]1.Selecting wax blocks of rats’HCC model induced by diethylnitrosamine (DEN) in the research group, including 15 cases of normal control,27 cases of liver cirrhosis,6 cases of cancer accompanied by cirrhosis,33 cases of liver cancer group with a total of 81 cases. Immunohistochemistry was used to detect AFP and DLK1 protein’s expression in liver cirrhosis and hepatocellular carcinoma.2. Screening and collecting blood of liver cancer, cirrhosis and normal people from Second Affiliated Hospital of Kunming Medical University between the year 2013-2014 in which there had normal for 8 cases,16 patients with liver cirrhosis,17 patients with hepatocellular carcinoma as a total of 41 cases, then taking serum separation and extraction. Using double-antibody ELISA sandwich method test DLK1 protein expression in human serum.[Result]1.Immunohistochemical test showed:AFP protein distributed in nested or scattered shape in the cell cytoplasm, there were rare expressions of extracellular matrix. Positive granules of DLK1 protein was mainly in the cytoplasm with the distribution of nested or scattered shape, there had expressions of extracellular matrix.The DLK1 and AFP no expression in normal group. The cirrhosis AFP positive expression was 37.04%(10/27), the DLKl positive expression was 29.63%(8/27). The AFP positive expression between HCC group and cirrhosis group had obvious difference which had statistically significant (P<0.05). The DLK1 positive expression differences also had a statistically significant (P<0.05). In cancer and cirrhosis group, the AFP positive expression was 100%(6/6) in carcinoma, the DLK1 positive expression was 66.67%(4/6).The AFP positive expression was 50%(3/6) in cancerous paraneoplastic nodules, the DLKl positive expression was 33.33%(2/6). AFP and DLK1 had no significance in pericancerous cirrhosis and without cancer cirrhosis group difference (P>0.05). The AFP positive expression in HCC group was 63.64%(21/33), the DLK1 positive expression was 57.58%(19/33), both differences of positive expressions didn’t have statistically significant (P> 0.05). Combined detection of these two expressions was 85.29% (28/33), which had statistically significant by comparing with comparison on single detections (P<0.05).ELISA results showedThe concentration of serum AFP in normal group (12.68 ±4.86ng/ml), cirrhosis group (63.34 ±10.32 ng/ml) and HCC group (368.21 ±134.14 ng/ml) compared to between the groups, the differences were statistically significant (P<0.05).The concentration of DLK1 in normal group serum (1712.7511 ±866.02259pg/ml), comparing with concentration of DLK1 in cirrhosis group serum (3855.0428 ± 3162.90487pg/ml), the comparative differences had statistically significant (P <0.05).The concentration level of serum in normal group was obvious lower than the DLK1 concentration in liver cancer group (5785.9240±1948.87716pg/ml) (P<0.01).There had no statistic meaning between the comparison on the concentration level of Serum in liver cirrhosis group and liver cancer group (P= 0.05).(Conclusion]1. The expression of AFP and DLK1 gradually increased in The process of hepatocarcinogenesis2. The two combined detection of liver cancer had more sensitivity and specificity than single detection.3. The sensitivity was weak in testing DLK1 serum concentration levels for HCC diagnosis.