The Effect Of OSW-1 And Deletion Of Mitochondrial DNA On PI3K Signaling Pathway In Hepatoma Carcinoma Cell

Objective:1. To study the effect of OSW-1 and deletion of mitochondrial DNA on PI3K signaling pathway in hepatoma carcinoma cell.Methods:1. Establish the mitochondrial DNA deletion model, p0-SK-Hep-1 (e.g.2), which was maked of the SK-Hep-1 cell(c.g.) as the control group. Compare the difference of expression between SK-Hep-1 andp0-SK-Hep-1 cell on PI3K signaling pathway by a quantitative real-time PCR Assay.2. SK-Hep-1 (e.g.1) and p0-SK-Hep-l(e.g.3) cell are intervened by OSW-124h, and compare the two groups’ different expression.Results:1. The up-regulated expression of experimental group 1 compares with control group:CASP9、CD1、FOXO1、GJA1、GRB2、HSPB1、IGF1、MAPK3、 PDPK1、PRKCA、RPS6KA1、TLR4、TSC2. The down-regulated expression of experimental group 1 compares with control group:APC、CTNNB1.2. the up-regulated expression of experimental group 2 compares with control group:FOS、 GRB2、HSPB1、IRAK1、PDPK1、PRKCA、PRKCB、TLR4、TOLLIP、TSC2. The down-regulated expression of experimental group 2 compares with control group: APC、IGF1、IRS1、PDGFRA、PTPN11、SHC1.3. The up-regulated expression of experimental group 3 compares with control group:AKT1、CDC42、CDKN1B、 FASLB、IRAK1、IRS1、PDGFRA、PDK2、PIK3R2、PRKCB、SHC1、TIRAP、 TOLLIP. The down-regulated expression of experimental group 3 compares with control group:BAD、EIF2AK2、FOXO1、IGF1、PABPC1、PAK1、PDK1、PDPK1、 PIK3CG、RAC1、RASA1、RHOA、SOS1、TLR4、WASL.4. The up-regulated expression of experimental group 1 and experimental group 3:IRAK1、PRKCB. The down-regulated expression of experimental group 1 and experimental group 3:IGF1、 APC.Conclusion:1. OSW-1 and deletion of mitochondrial DNA could effect the expression of PI3K signaling pathway in SK-Hep-1 cell.2. The targeting effects of OSW-1 on the mitochondria of SK-Hep-1 was proved.3. OSW-1 inhibited IGF-1-PI3K-AKT, Grb2-p85-AKT, IRS1-PI3K-AKT-GLUT-4, PI3K-AKT-TSC1/ TSC2- mTOR and TLR4-PI3K-AKT signaling pathway to inhibit hepatoma carcinoma cell’s growth, multiplication, energy metabolism.