Polymorphism Of Haptoglobin In Patients With Premature Rupture Of Membrane

Objective: Premature rupture of membrane(PROM) is one of the commom obstetrical complications that are posing a health risk for mothers and infants. Although there are many studies of the pathogenesis of premature rupture of membranes, but the Etiology and pathogenesis of it is unclear now. To reduce the risk of fetal and maternal complications, it is required to find specific biomolecules associated with development of PROM. Genetic factor plays an important role in many diseases. A variety of susceptibility genes have been found. But study of susceptibility genes for premature rupture of membranes is still in exploration. Haptoglobin(Hp) that synthesizes primarily by hepatocytes is a plasma α2-glycoprotein. As an acute phase protein Hp plays a role in the systemic host defense in response to inflammation and injury. It binds hemoglobin(Hb), thereby protecting cells from oxidative damage. Hp is considered to have functions as antioxidant, immunomodulator and angiogenic factor. In addition, Hp exerts a stimulatory effect on differentiation of endothelial cells and an inhibitory effect on prostaglandin synthesis. The human Hp gene has 2 major alleles, Hp1 and Hp2, which are expressed as 3 major phenotypes(Hp 1-1, Hp 2-2 and Hp 2-1). Hp proteins with the 3 phenotypes show phenotype-dependent functional differences in anti-oxidant, anti-inflammatory and angiogenic activities. To investigate whether allelic polymorphism of Hp is associated with PPROM and tPROM, the Hp phenotypes of pregnant women with PROM were analyzed. Methods: 49 pregnant women with PROM selected randomly from March 1, 2014 to August 31, 2014 in the first affiliated hospital of Wannan Medical College as experimental group. Membrane rupture before 37 weeks of gestation is served as PPROM group and membrane rupture after 37 weeks of gestation is served as PROM group. 32 Term pregnant women without obstetric complications and medical and surgery complication were selected as control group in the same period. Blood was collected from each patient on admission. Subjects blood DNA was extracted from the peripheral blood by Wizard ® Genomic DNA Purification Kit providing by Promege. Reference by Wemer Koch,the PCR—SSP technology was used to explore the Hp genotype. Results: 1. As for Hp polymorphism, the pregnant women with control group show Hp 1-1, 2-1, and 2-2 phenotypes at 21.88%, 34.37% and 43.75%, respectively. In contrast patients with PPROM demonstrate Hp 1-1, 2-1, and 2-2 phenotypes at 16.00%, 24.00% and 60.00%, respectively. The frequency of Hp2 in control group is 0.6094 and in PPROM group is 0.7200. There is no statistically significant difference between PPROM group and control group. 2. Patients with PROM show Hp 1-1, 2-1, and 2-2 phenotypes at 16.00%, 24.00% and 60.00%, respectively. Women with PROM have a similar allele frequency(0.6667) with control women(0.6094). There is no statistically significant difference between PROM group and control group. Conclusion: Polymorphism of Haptoglobin may not be the risk factors of preterm premature rupture of membrane and term premature rupture of membrane in Wu Hu, An Hui, China.